Hotspot

trnI-CAU-trnF-GAA

Genome · 66023-67736 · 1714 bp

Back to Pinus

Pi 0.000292
MarkerSeek score 58.90
Primer available yes
Label trnI-CAU-trnF-GAA

Feature Metadata

label_name
trnI-CAU-trnF-GAA
feature_type
igs
region
Genome
length_bp
1714
variable_sites
1
pi
0.000292
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-trnF-GAA_p1 GTTGGGCGCTTTAACCATTC AGGACTGAAAATCCTCGTGC 50.0 50.0 58.0 57.9 1788-1796 bp 1.0 79.4
trnI-CAU-trnF-GAA_p2 GGATATGTGCAGTTCCCCTG AGGACTGAAAATCCTCGTGC 55.0 50.0 58.0 57.9 1848-1856 bp 1.0 79.2
trnI-CAU-trnF-GAA_p3 GTTGGGCGCTTTAACCATTC GACTGAAAATCCTCGTGCCA 50.0 50.0 58.0 58.2 1786-1794 bp 1.0 78.7
trnI-CAU-trnF-GAA_p4 GTTGGGCGCTTTAACCATTC TACCAGGATGATCCACAGGG 50.0 55.0 58.0 58.2 1839-1847 bp 1.0 78.6
trnI-CAU-trnF-GAA_p5 GGATATGTGCAGTTCCCCTG GACTGAAAATCCTCGTGCCA 55.0 50.0 58.0 58.2 1846-1854 bp 1.0 78.5