Hotspot

cemA-ycf4

Genome · 39064-39730 · 667 bp

Back to Pinus

Pi 0.0045
MarkerSeek score 47.80
Primer available yes
Label cemA-ycf4

Feature Metadata

label_name
cemA-ycf4
feature_type
igs
region
Genome
length_bp
667
variable_sites
6
pi
0.0045
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA-ycf4_p1 CGGACACTTCCAATTCGTGA TGCGTGTATCCATTGAAGGG 50.0 50.0 58.2 58.0 796 bp 1.0 78.6
cemA-ycf4_p2 TCGGACACTTCCAATTCGTG TGCGTGTATCCATTGAAGGG 50.0 50.0 58.2 58.0 797 bp 1.0 78.6
cemA-ycf4_p3 CGGACACTTCCAATTCGTGA GCGTGTATCCATTGAAGGGT 50.0 50.0 58.2 58.0 795 bp 1.0 78.6
cemA-ycf4_p4 TCGGACACTTCCAATTCGTG GCGTGTATCCATTGAAGGGT 50.0 50.0 58.2 58.0 796 bp 1.0 78.6
cemA-ycf4_p5 CGGACACTTCCAATTCGTGA AACAGAAAGCCGCTGAATCA 50.0 45.0 58.2 58.1 826 bp 1.0 78.1