Hotspot

psbJ-petA

Genome · 36098-37088 · 991 bp

Back to Pinus

Pi 0.00152
MarkerSeek score 60.00
Primer available yes
Label psbJ-petA

Feature Metadata

label_name
psbJ-petA
feature_type
igs
region
Genome
length_bp
991
variable_sites
3
pi
0.00152
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbJ-petA_p1 CGTGATTGGTCTACTGGGTG AGCATCTGTCATTCTGGCAC 55.0 50.0 58.0 58.3 1140-1159 bp 1.0 78.2
psbJ-petA_p2 TGGAAGGATCCCTCTTTGGT AGCATCTGTCATTCTGGCAC 50.0 50.0 57.9 58.3 1185-1204 bp 1.0 77.8
psbJ-petA_p3 TCGTGATTGGTCTACTGGGT AGCATCTGTCATTCTGGCAC 50.0 50.0 58.1 58.3 1141-1160 bp 1.0 77.8
psbJ-petA_p4 TACCACTGGAAGGATCCCTC AGCATCTGTCATTCTGGCAC 55.0 50.0 57.8 58.3 1191-1210 bp 1.0 76.9
psbJ-petA_p5 CGTGATTGGTCTACTGGGTG GCATCTGTCATTCTGGCACA 55.0 50.0 58.0 58.5 1139-1158 bp 1.0 75.9