Hotspot

psbA-trnK-UUU

Genome · 1063-1596 · 534 bp

Back to Pinus

Pi 0.000938
MarkerSeek score 59.00
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
Genome
length_bp
534
variable_sites
1
pi
0.000938
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 GGTAGGGATCATCAAGACGC AGAATTGCAAGCACGGTTTG 55.0 45.0 57.8 57.9 756-762 bp 1.0 78.3
psbA-trnK-UUU_p2 GTAGGGATCATCAAGACGCC AGAATTGCAAGCACGGTTTG 55.0 45.0 57.8 57.9 755-761 bp 1.0 78.3
psbA-trnK-UUU_p3 GGTAGGGATCATCAAGACGC AGAGCAGATCATCCACTCCA 55.0 50.0 57.8 57.8 705-711 bp 1.0 78.1
psbA-trnK-UUU_p4 GTAGGGATCATCAAGACGCC AGAGCAGATCATCCACTCCA 55.0 50.0 57.8 57.8 704-710 bp 1.0 78.1
psbA-trnK-UUU_p5 GTAGGGATCATCAAGACGCC GCAGATCATCCACTCCATCC 55.0 55.0 57.8 57.8 701-707 bp 1.0 78.0