primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	GGTAGGGATCATCAAGACGC	AGAATTGCAAGCACGGTTTG	20	20	55.0	45.0	57.8	57.9	0.0	4.9	0.294939	1063	1596	756-762	1.000000	78.3
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	GTAGGGATCATCAAGACGCC	AGAATTGCAAGCACGGTTTG	20	20	55.0	45.0	57.8	57.9	0.0	4.9	0.294939	1063	1596	755-761	1.000000	78.3
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	GGTAGGGATCATCAAGACGC	AGAGCAGATCATCCACTCCA	20	20	55.0	50.0	57.8	57.8	0.0	0.0	0.327911	1063	1596	705-711	1.000000	78.1
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	GTAGGGATCATCAAGACGCC	AGAGCAGATCATCCACTCCA	20	20	55.0	50.0	57.8	57.8	0.0	0.0	0.327911	1063	1596	704-710	1.000000	78.1
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	GTAGGGATCATCAAGACGCC	GCAGATCATCCACTCCATCC	20	20	55.0	55.0	57.8	57.8	0.0	0.0	0.338297	1063	1596	701-707	1.000000	78.0
psaM_p1	psaM	1	CCAGGCCAAGAAAAGCAGAA	ACTCATTGGGGAGGGAATGA	20	20	50.0	50.0	58.7	58.0	5.0	0.0	0.683165	8299	8391	141-144	1.000000	79.6
psaM_p2	psaM	2	CCAGGCCAAGAAAAGCAGAA	TCAAACTCATTGGGGAGGGA	20	20	50.0	50.0	58.7	58.2	5.0	0.0	0.914884	8299	8391	145-148	1.000000	77.6
psaM_p3	psaM	3	CCAGGCCAAGAAAAGCAGA	ACTCATTGGGGAGGGAATGA	19	20	52.6	50.0	58.0	58.0	5.0	0.0	1.055344	8299	8391	141-144	1.000000	76.6
psaM_p4	psaM	4	CAGGCCAAGAAAAGCAGAAA	ACTCATTGGGGAGGGAATGA	20	20	45.0	50.0	56.8	58.0	5.0	0.0	1.198688	8299	8391	140-143	1.000000	75.5
psaM_p5	psaM	5	CCAGGCCAAGAAAAGCAGAA	CAAACTCATTGGGGAGGGAA	20	20	50.0	50.0	58.7	57.4	5.0	0.0	1.271861	8299	8391	144-147	1.000000	74.8
psaM-ycf12_p1	psaM-ycf12	1	TCCGTAGAAAGATTCGGGGT	CTAAAGGGCCCGATACAACC	20	20	50.0	55.0	58.1	58.0	0.0	18.4	0.180199	8392	8684	813-816	1.000000	79.7
psaM-ycf12_p2	psaM-ycf12	2	TCCGTAGAAAGATTCGGGGT	AGCTATTCGGCTTCAACCTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.173209	8392	8684	1324-1327	1.000000	79.4
psaM-ycf12_p3	psaM-ycf12	3	TCCGTAGAAAGATTCGGGGT	CCGCCACGATCTGATTGTAA	20	20	50.0	50.0	58.1	58.1	0.0	10.1	0.208782	8392	8684	1004-1007	1.000000	79.4
psaM-ycf12_p4	psaM-ycf12	4	TCCGTAGAAAGATTCGGGGT	TGAGCTGCGCAATAACTTCT	20	20	50.0	45.0	58.1	57.9	0.0	13.1	0.242512	8392	8684	777-780	1.000000	79.3
psaM-ycf12_p5	psaM-ycf12	5	TCCGTAGAAAGATTCGGGGT	TATACCCGCCACGATCTGAT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.224131	8392	8684	1009-1012	1.000000	79.2
trnG-UCC_p1	trnG-UCC	1	GGTTGGACAAAAGATCGATCC	CGAGTGAATCGTGAAATTATGT	21	22	47.6	36.4	57.0	55.0	21.7	0.0	7.074439	8936	9745	947-949	1.000000	40.3
trnG-UCC_p2	trnG-UCC	2	GGTTGGACAAAAGATCGATCC	CGAGTGAATCGTGAAATTATGTT	21	23	47.6	34.8	57.0	55.7	21.7	0.0	7.341780	8936	9745	947-949	1.000000	40.3
trnG-UCC_p3	trnG-UCC	3	GGTTGGACAAAAGATCGATCCA	CGAGTGAATCGTGAAATTATGT	22	22	45.5	36.4	58.7	55.0	21.7	0.0	7.692922	8936	9745	947-949	1.000000	40.3
trnG-UCC_p4	trnG-UCC	4	TGGTTGGACAAAAGATCGATCC	CGAGTGAATCGTGAAATTATGT	22	22	45.5	36.4	58.7	55.0	21.7	0.0	7.692922	8936	9745	948-950	1.000000	40.3
trnG-UCC_p5	trnG-UCC	5	GTTGGACAAAAGATCGATCCA	CGAGTGAATCGTGAAATTATGT	21	22	42.9	36.4	56.3	55.0	21.7	0.0	7.782139	8936	9745	946-948	1.000000	40.3
atpH-atpI_p1	atpH-atpI	1	AATGGAAGCAAGCCCTACAG	TCTTTGCAACTCTAGCTGCA	20	20	50.0	45.0	57.9	57.5	0.0	0.0	0.683853	13622	14312	813-813	1.000000	74.9
atpH-atpI_p2	atpH-atpI	2	CAATGGAAGCAAGCCCTACA	TCTTTGCAACTCTAGCTGCA	20	20	50.0	45.0	58.2	57.5	0.0	0.0	0.710951	13622	14312	814-814	1.000000	74.7
atpH-atpI_p3	atpH-atpI	3	AATGGAAGCAAGCCCTACAG	TAGGTGAATCCATGGAGGGT	20	20	50.0	50.0	57.9	57.4	0.0	21.3	0.721849	13622	14312	786-786	1.000000	74.6
atpH-atpI_p4	atpH-atpI	4	CAATGGAAGCAAGCCCTACA	TAGGTGAATCCATGGAGGGT	20	20	50.0	50.0	58.2	57.4	0.0	21.3	0.748948	13622	14312	787-787	1.000000	74.4
atpH-atpI_p5	atpH-atpI	5	TCCAGCAGCAATAACGGAAG	TCTTTGCAACTCTAGCTGCA	20	20	50.0	45.0	58.3	57.5	0.0	0.0	0.811225	13622	14312	789-789	1.000000	73.9
petN-psbM_p1	petN-psbM	1	CGGTATAACTTGGGCTGCTT	GCAAGCAGTGGAAGCAATTG	20	20	50.0	50.0	58.0	58.6	0.0	0.0	0.612975	26939	27833	989-995	1.000000	75.8
petN-psbM_p2	petN-psbM	2	GGGGGAGAAGTGGGCTATAA	GCAAGCAGTGGAAGCAATTG	20	20	55.0	50.0	58.2	58.6	0.0	0.0	0.758831	26939	27833	930-936	1.000000	74.7
petN-psbM_p3	petN-psbM	3	ACTTGGGCTGCTTTAATGGT	GCAAGCAGTGGAAGCAATTG	20	20	45.0	50.0	57.7	58.6	0.0	0.0	0.883724	26939	27833	982-988	1.000000	73.7
petN-psbM_p4	petN-psbM	4	CACTCGTAGTATGGGGGAGA	GCAAGCAGTGGAAGCAATTG	20	20	55.0	50.0	57.6	58.6	0.0	0.0	0.934569	26939	27833	942-948	1.000000	73.3
petN-psbM_p5	petN-psbM	5	TCACTCGTAGTATGGGGGAG	GCAAGCAGTGGAAGCAATTG	20	20	55.0	50.0	57.6	58.6	0.0	0.0	0.934569	26939	27833	943-949	1.000000	73.3
trnE-UUC-clpP_p1	trnE-UUC-clpP	1	ATCCACTAGACGATAGGGGC	GGCACTTTTGGAACACCAAC	20	20	55.0	50.0	58.1	58.1	13.7	0.0	0.139303	29019	29641	642-716	1.000000	82.4
trnE-UUC-clpP_p2	trnE-UUC-clpP	2	ATCCACTAGACGATAGGGGC	AGGCACTTTTGGAACACCAA	20	20	55.0	45.0	58.1	58.1	13.7	0.0	0.214590	29019	29641	643-717	1.000000	81.8
trnE-UUC-clpP_p3	trnE-UUC-clpP	3	CGTTGCCTCCTTGAAAGAGA	GGCACTTTTGGAACACCAAC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.235244	29019	29641	671-745	1.000000	81.5
trnE-UUC-clpP_p4	trnE-UUC-clpP	4	CGAATGGATCATGGTACCCC	GGCACTTTTGGAACACCAAC	20	20	55.0	50.0	57.8	58.1	10.1	0.0	0.250415	29019	29641	709-783	1.000000	81.2
trnE-UUC-clpP_p5	trnE-UUC-clpP	5	ATCCACTAGACGATAGGGGC	TCGACCCAAGTTGCATCTTC	20	20	55.0	50.0	58.1	58.2	13.7	7.1	0.265481	29019	29641	681-755	1.000000	81.2
rps18_p1	rps18	1	GCAGTAGCAAAAGCCATTGG	TATGGCCAAGAGCGGAGATA	20	20	50.0	50.0	58.0	58.0	0.0	11.0	0.010111	31851	32153	1369-1369	1.000000	80.2
rps18_p2	rps18	2	GCAGTAGCAAAAGCCATTGG	TGGCCAAGAGCGGAGATATA	20	20	50.0	50.0	58.0	58.0	0.0	0.6	0.010111	31851	32153	1367-1367	1.000000	80.2
rps18_p3	rps18	3	AAGAGGCAGTTGCTTCCAAA	TATGGCCAAGAGCGGAGATA	20	20	45.0	50.0	57.9	58.0	12.0	11.0	0.081459	31851	32153	992-992	1.000000	79.7
rps18_p4	rps18	4	AAGAGGCAGTTGCTTCCAAA	TGGCCAAGAGCGGAGATATA	20	20	45.0	50.0	57.9	58.0	12.0	0.6	0.081459	31851	32153	990-990	1.000000	79.7
rps18_p5	rps18	5	GCAGTAGCAAAAGCCATTGG	GGACTGGAAGGGGGTAGAAT	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.105730	31851	32153	1579-1579	1.000000	79.5
psbJ-petA_p1	psbJ-petA	1	CGTGATTGGTCTACTGGGTG	AGCATCTGTCATTCTGGCAC	20	20	55.0	50.0	58.0	58.3	0.0	0.0	0.262436	36098	37088	1140-1159	1.000000	78.2
psbJ-petA_p2	psbJ-petA	2	TGGAAGGATCCCTCTTTGGT	AGCATCTGTCATTCTGGCAC	20	20	50.0	50.0	57.9	58.3	8.6	0.0	0.312047	36098	37088	1185-1204	1.000000	77.8
psbJ-petA_p3	psbJ-petA	3	TCGTGATTGGTCTACTGGGT	AGCATCTGTCATTCTGGCAC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.319269	36098	37088	1141-1160	1.000000	77.8
psbJ-petA_p4	psbJ-petA	4	TACCACTGGAAGGATCCCTC	AGCATCTGTCATTCTGGCAC	20	20	55.0	50.0	57.8	58.3	8.6	0.0	0.428538	36098	37088	1191-1210	1.000000	76.9
psbJ-petA_p5	psbJ-petA	5	CGTGATTGGTCTACTGGGTG	GCATCTGTCATTCTGGCACA	20	20	55.0	50.0	58.0	58.5	0.0	0.0	0.553849	36098	37088	1139-1158	1.000000	75.9
cemA-ycf4_p1	cemA-ycf4	1	CGGACACTTCCAATTCGTGA	TGCGTGTATCCATTGAAGGG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.246202	39064	39730	796-796	1.000000	78.6
cemA-ycf4_p2	cemA-ycf4	2	TCGGACACTTCCAATTCGTG	TGCGTGTATCCATTGAAGGG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.246202	39064	39730	797-797	1.000000	78.6
cemA-ycf4_p3	cemA-ycf4	3	CGGACACTTCCAATTCGTGA	GCGTGTATCCATTGAAGGGT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.247632	39064	39730	795-795	1.000000	78.6
cemA-ycf4_p4	cemA-ycf4	4	TCGGACACTTCCAATTCGTG	GCGTGTATCCATTGAAGGGT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.247632	39064	39730	796-796	1.000000	78.6
cemA-ycf4_p5	cemA-ycf4	5	CGGACACTTCCAATTCGTGA	AACAGAAAGCCGCTGAATCA	20	20	50.0	45.0	58.2	58.1	0.0	0.0	0.313025	39064	39730	826-826	1.000000	78.1
trnI-CAU-trnF-GAA_p1	trnI-CAU-trnF-GAA	1	GTTGGGCGCTTTAACCATTC	AGGACTGAAAATCCTCGTGC	20	20	50.0	50.0	58.0	57.9	7.6	0.0	0.109805	66023	67736	1788-1796	1.000000	79.4
trnI-CAU-trnF-GAA_p2	trnI-CAU-trnF-GAA	2	GGATATGTGCAGTTCCCCTG	AGGACTGAAAATCCTCGTGC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.127733	66023	67736	1848-1856	1.000000	79.2
trnI-CAU-trnF-GAA_p3	trnI-CAU-trnF-GAA	3	GTTGGGCGCTTTAACCATTC	GACTGAAAATCCTCGTGCCA	20	20	50.0	50.0	58.0	58.2	7.6	0.0	0.200790	66023	67736	1786-1794	1.000000	78.7
trnI-CAU-trnF-GAA_p4	trnI-CAU-trnF-GAA	4	GTTGGGCGCTTTAACCATTC	TACCAGGATGATCCACAGGG	20	20	50.0	55.0	58.0	58.2	7.6	14.9	0.202705	66023	67736	1839-1847	1.000000	78.6
trnI-CAU-trnF-GAA_p5	trnI-CAU-trnF-GAA	5	GGATATGTGCAGTTCCCCTG	GACTGAAAATCCTCGTGCCA	20	20	55.0	50.0	58.0	58.2	0.0	0.0	0.218717	66023	67736	1846-1854	1.000000	78.5
rps4_p1	rps4	1	TCCAGTAGTACTCATACGGGT	AATTTCCAGATGCCCAGCAA	21	20	47.6	45.0	57.1	58.1	11.7	0.0	1.998362	69543	70148	726-735	1.000000	65.4
rps4_p2	rps4	2	TCCAGTAGTACTCATACGGGT	GGGGAAGGGAAAAAGAAGGG	21	20	47.6	55.0	57.1	58.1	11.7	0.0	2.001155	69543	70148	756-765	1.000000	65.4
rps4_p3	rps4	3	TCCAGTAGTACTCATACGGGT	AACCCACCCCTCTCTTTCAT	21	20	47.6	50.0	57.1	58.2	11.7	0.0	2.179335	69543	70148	704-713	1.000000	64.0
rps4_p4	rps4	4	TCCAGTAGTACTCATACGGGT	ACCCACCCCTCTCTTTCATT	21	20	47.6	50.0	57.1	58.2	11.7	0.0	2.179335	69543	70148	703-712	1.000000	64.0
rps4_p5	rps4	5	TCCAGTAGTACTCATACGGGTT	AATTTCCAGATGCCCAGCAA	22	20	45.5	45.0	57.8	58.1	11.7	0.0	2.299504	69543	70148	726-735	1.000000	63.0
ycf1_p1	ycf1	1	ATGTGCTTCTGATGGTACCG	TGGAACGCGTCTTATTCCTG	20	20	50.0	50.0	57.7	58.0	7.6	23.3	0.320508	98232	98351	292-292	1.000000	77.7
ycf1_p2	ycf1	2	ATGTGCTTCTGATGGTACCG	GGAACGCGTCTTATTCCTGT	20	20	50.0	50.0	57.7	58.0	7.6	14.4	0.321896	98232	98351	291-291	1.000000	77.7
ycf1_p3	ycf1	3	ATGTGCTTCTGATGGTACCG	GTGGAACGCGTCTTATTCCT	20	20	50.0	50.0	57.7	58.0	7.6	23.3	0.321896	98232	98351	293-293	1.000000	77.7
ycf1_p4	ycf1	4	GTGCTTCTGATGGTACCGAA	TGGAACGCGTCTTATTCCTG	20	20	50.0	50.0	57.6	58.0	7.6	23.3	0.384666	98232	98351	290-290	1.000000	77.2
ycf1_p5	ycf1	5	GTGCTTCTGATGGTACCGAA	GGAACGCGTCTTATTCCTGT	20	20	50.0	50.0	57.6	58.0	7.6	14.4	0.386054	98232	98351	289-289	1.000000	77.2
rps15-psaC_p1	rps15-psaC	1	GGTCAATTGGGTATCCGTGG	GTACGCATTGGGTACAACCA	20	20	55.0	50.0	58.3	58.2	5.4	29.0	0.513489	101422	103539	2208-2209	1.000000	76.0
rps15-psaC_p2	rps15-psaC	2	GTCAATTGGGTATCCGTGGG	GTACGCATTGGGTACAACCA	20	20	55.0	50.0	58.3	58.2	5.4	29.0	0.513489	101422	103539	2207-2208	1.000000	76.0
rps15-psaC_p3	rps15-psaC	3	TGGTCAATTGGGTATCCGTG	GTACGCATTGGGTACAACCA	20	20	50.0	50.0	57.6	58.2	5.4	29.0	0.600669	101422	103539	2209-2210	1.000000	75.3
rps15-psaC_p4	rps15-psaC	4	GGGTATCCGTGGGCTAAAAA	GTACGCATTGGGTACAACCA	20	20	50.0	50.0	57.6	58.2	0.0	29.0	0.612487	101422	103539	2200-2201	1.000000	75.2
rps15-psaC_p5	rps15-psaC	5	ATTGGGTATCCGTGGGCTAA	GTACGCATTGGGTACAACCA	20	20	50.0	50.0	58.5	58.2	0.0	29.0	0.675183	101422	103539	2203-2204	1.000000	74.7
psaC-ccsA_p1	psaC-ccsA	1	GAATCCGCTTGTCCAACAGA	GGATCCGTTATTTGGGGGTC	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.214533	103786	104943	1310-1311	1.000000	78.9
psaC-ccsA_p2	psaC-ccsA	2	TCCGCTTGTCCAACAGATTT	GGATCCGTTATTTGGGGGTC	20	20	45.0	55.0	57.7	58.0	0.0	0.0	0.293296	103786	104943	1307-1308	1.000000	78.3
psaC-ccsA_p3	psaC-ccsA	3	CAGGTTGTAAGCGGTGTGAA	GGATCCGTTATTTGGGGGTC	20	20	50.0	55.0	58.4	58.0	0.0	0.0	0.434270	103786	104943	1327-1328	1.000000	77.1
psaC-ccsA_p4	psaC-ccsA	4	TGAATCCGCTTGTCCAACAG	GGATCCGTTATTTGGGGGTC	20	20	50.0	55.0	58.5	58.0	0.0	0.0	0.500276	103786	104943	1311-1312	1.000000	76.6
psaC-ccsA_p5	psaC-ccsA	5	AAGACTGCGCAGGTTGTAAG	GGATCCGTTATTTGGGGGTC	20	20	50.0	55.0	58.5	58.0	38.3	0.0	0.510018	103786	104943	1336-1337	1.000000	76.5
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	GTCAAGGTGACACTCTACCG	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	57.7	57.7	5.4	16.5	0.606988	108342	109723	1462-1465	1.000000	75.4
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	GGCTCGAACTGATGACTTCC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.4	57.7	0.0	16.5	0.611502	108342	109723	1487-1490	1.000000	75.3
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	GCTCGAACTGATGACTTCCA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.6	57.7	0.0	16.5	0.627972	108342	109723	1486-1489	1.000000	75.2
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	CAGGCTCGAACTGATGACTT	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.6	57.7	10.4	16.5	0.634499	108342	109723	1489-1492	1.000000	75.1
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	TGACACTCTACCGCTGAGTT	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	58.4	57.7	0.0	16.5	0.640368	108342	109723	1455-1458	1.000000	75.1
ycf2_p1	ycf2	1	ATGGCTGCATACGTTCCAAT	CGCCGAGAATTTTTCGCAAA	20	20	45.0	45.0	57.9	58.3	0.5	3.9	0.376469	112992	113111	256-256	1.000000	77.6
ycf2_p2	ycf2	2	TGGCTGCATACGTTCCAATT	CGCCGAGAATTTTTCGCAAA	20	20	45.0	45.0	58.2	58.3	0.5	3.9	0.486448	112992	113111	255-255	1.000000	76.7
ycf2_p3	ycf2	3	GCTGCATACGTTCCAATTGG	CGCCGAGAATTTTTCGCAAA	20	20	50.0	45.0	57.8	58.3	19.1	3.9	0.512638	112992	113111	253-253	1.000000	76.5
ycf2_p4	ycf2	4	GGCTGCATACGTTCCAATTG	CGCCGAGAATTTTTCGCAAA	20	20	50.0	45.0	57.8	58.3	0.5	3.9	0.512638	112992	113111	254-254	1.000000	76.5
ycf2_p5	ycf2	5	ATTTTCGGTCAACCAACGGT	CGCCGAGAATTTTTCGCAAA	20	20	45.0	45.0	58.3	58.3	0.0	3.9	0.636592	112992	113111	297-297	1.000000	75.4