Hotspot

ycf1-rpl32

SSC · 108043-108513 · 471 bp

Back to Pinguicula

Pi 0.104

MarkerSeek score 66.90

Primer available yes

Label ycf1-rpl32

Feature Metadata

label_name: ycf1-rpl32
feature_type: igs
region: SSC
length_bp: 471
variable_sites: 71
pi: 0.104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rpl32_p1	TAAAGCTTTGGACAGGGCTG	TGAATTCCCGGTAGAAAGCG	50.0	50.0	58.1	58.0	1237 bp	0.333333	52.5
ycf1-rpl32_p2	TTGGACAGGGCTGAAATGTC	TGAATTCCCGGTAGAAAGCG	50.0	50.0	58.1	58.0	1230 bp	0.333333	52.5
ycf1-rpl32_p3	GACAGGGCTGAAATGTCACA	TGAATTCCCGGTAGAAAGCG	50.0	50.0	58.1	58.0	1227 bp	0.333333	52.4
ycf1-rpl32_p4	TTGGACAGGGCTGAAATGTC	CCAAGAACCCTCCCTTTTCC	50.0	55.0	58.1	58.1	1179 bp	0.333333	52.0
ycf1-rpl32_p5	TTGGACAGGGCTGAAATGTC	CCCAAGAACCCTCCCTTTTC	50.0	55.0	58.1	58.1	1180 bp	0.333333	52.0