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psbE-petL

LSC · 62960-63839 · 880 bp

Back to Pinguicula

Pi 0.0197

MarkerSeek score 72.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 25
pi: 0.0197
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAAACCGA	50.0	45.0	57.8	55.9	949-950 bp	1.0	65.4
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	AGCCACTAGTAGAAAACCGA	55.0	45.0	58.2	55.9	944-945 bp	1.0	65.1
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AGCCACTAGTAGAAAACCGA	50.0	45.0	58.4	55.9	942-943 bp	1.0	63.9
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACT	50.0	45.0	57.8	55.7	996-997 bp	1.0	63.7
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAAACCGAA	50.0	42.9	57.8	56.7	949-950 bp	1.0	63.6