Hotspot

accD-psaI

LSC · 57271-57739 · 469 bp

Back to Pinguicula

Pi 0.082

MarkerSeek score 72.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 469
variable_sites: 52
pi: 0.082
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TAAGCTCCACGCCTTCTTTC	AAAGAAGCCATCGCAATTGC	50.0	45.0	57.9	58.0	553-592 bp	1.0	90.6
accD-psaI_p2	CTCCACGCCTTCTTTCCTTT	AAAGAAGCCATCGCAATTGC	50.0	45.0	58.1	58.0	549-588 bp	1.0	90.6
accD-psaI_p3	TAAGCTCCACGCCTTCTTTC	TCGCAATTGCCGGAAATACT	50.0	45.0	57.9	58.3	543-582 bp	1.0	88.7
accD-psaI_p4	CTCCACGCCTTCTTTCCTTT	TCGCAATTGCCGGAAATACT	50.0	45.0	58.1	58.3	539-578 bp	1.0	88.7
accD-psaI_p5	CCACGCCTTCTTTCCTTTGA	AAAGAAGCCATCGCAATTGC	50.0	45.0	58.4	58.0	547-586 bp	1.0	88.3