Hotspot

trnT-GGU-psbD

LSC · 31472-32824 · 1353 bp

Back to Pinguicula

Pi 0.0679

MarkerSeek score 78.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1353
variable_sites: 130
pi: 0.0679
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAGAGCAATAGGCCAGACC	55.0	55.0	58.1	58.0	1505-1528 bp	1.0	90.0
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GGAAGAGCAATAGGCCAGAC	55.0	55.0	58.1	58.0	1506-1529 bp	1.0	90.0
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	GAAGAGCAATAGGCCAGACC	50.0	55.0	58.1	58.0	1508-1531 bp	1.0	89.9
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GGAAGAGCAATAGGCCAGAC	50.0	55.0	58.1	58.0	1509-1532 bp	1.0	89.9
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTG	55.0	50.0	58.1	57.9	1479-1502 bp	1.0	89.6