Hotspot

psbK-psbI

LSC · 7351-7738 · 388 bp

Back to Pinguicula

Pi 0.0262

MarkerSeek score 66.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 388
variable_sites: 15
pi: 0.0262
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	GATTACGCCCCGGATCATTT	50.0	50.0	57.9	58.1	512-515 bp	1.0	82.3
psbK-psbI_p2	CTTTTGTTTGGCAAGCTGCT	GATTACGCCCCGGATCATTT	45.0	50.0	58.1	58.1	517-520 bp	1.0	82.2
psbK-psbI_p3	CTTTTGTTTGGCAAGCTGCT	GCCCCGGATCATTTGATAGG	45.0	55.0	58.1	58.2	511-514 bp	1.0	81.8
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	GCCCCGGATCATTTGATAGG	50.0	55.0	57.9	58.2	506-509 bp	1.0	81.8
psbK-psbI_p5	GCCAGTCATACCTGTGCTTT	GATTACGCCCCGGATCATTT	50.0	50.0	58.2	58.1	549-552 bp	1.0	81.1