primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CAATCCACTGCCTTGATCCA	GAGCTTAGTTTCCGTCTGGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	84	450	971-1069	1.000000	86.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	84	450	977-1075	1.000000	86.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GGAGCTTAGTTTCCGTCTGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	84	450	972-1070	1.000000	86.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CAATCCACTGCCTTGATCCA	GGGAGCTTAGTTTCCGTCTG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.144821	84	450	973-1071	1.000000	86.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	AATCCACTGCCTTGATCCAC	GAGCTTAGTTTCCGTCTGGG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.146329	84	450	970-1068	1.000000	86.5
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGCACTTAAAAGCCGAGTA	TCCTTGAGAAAGGTGCTCAAC	20	21	50.0	47.6	58.3	58.1	5.3	0.0	1.431611	4343	5060	847-1032	1.000000	70.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TTCAAGCCGCACTTAAAAGC	TCCTTGAGAAAGGTGCTCAAC	20	21	45.0	47.6	57.6	58.1	0.0	0.0	1.564502	4343	5060	853-1038	1.000000	69.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	TCCTTGAGAAAGGTGCTCAA	20	20	50.0	45.0	58.3	56.7	5.3	0.0	1.605668	4343	5060	847-1032	1.000000	69.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	TTCCTTGAGAAAGGTGCTCA	20	20	50.0	45.0	58.3	56.7	5.3	4.5	1.605668	4343	5060	848-1033	1.000000	69.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGCACTTAAAAGCCGAGTA	CCTTGAGAAAGGTGCTCAAC	20	20	50.0	50.0	58.3	56.6	5.3	0.0	1.643000	4343	5060	846-1031	1.000000	69.0
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	GATTACGCCCCGGATCATTT	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.163885	7351	7738	512-515	1.000000	82.3
psbK-psbI_p2	psbK-psbI	2	CTTTTGTTTGGCAAGCTGCT	GATTACGCCCCGGATCATTT	20	20	45.0	50.0	58.1	58.1	15.2	0.7	0.163759	7351	7738	517-520	1.000000	82.2
psbK-psbI_p3	psbK-psbI	3	CTTTTGTTTGGCAAGCTGCT	GCCCCGGATCATTTGATAGG	20	20	45.0	55.0	58.1	58.2	15.2	0.7	0.229584	7351	7738	511-514	1.000000	81.8
psbK-psbI_p4	psbK-psbI	4	GTTTGGCAAGCTGCTGTAAG	GCCCCGGATCATTTGATAGG	20	20	50.0	55.0	57.9	58.2	1.4	0.7	0.229709	7351	7738	506-509	1.000000	81.8
psbK-psbI_p5	psbK-psbI	5	GCCAGTCATACCTGTGCTTT	GATTACGCCCCGGATCATTT	20	20	50.0	50.0	58.2	58.1	0.0	0.7	0.281406	7351	7738	549-552	1.000000	81.1
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GAAGAGCAATAGGCCAGACC	20	20	55.0	55.0	58.1	58.0	7.6	5.0	0.103651	31472	32824	1505-1528	1.000000	90.0
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GTGGTAGAGTAACGCCATGG	GGAAGAGCAATAGGCCAGAC	20	20	55.0	55.0	58.1	58.0	7.6	5.0	0.103651	31472	32824	1506-1529	1.000000	90.0
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TCAGTGGTAGAGTAACGCCA	GAAGAGCAATAGGCCAGACC	20	20	50.0	55.0	58.1	58.0	0.0	5.0	0.125805	31472	32824	1508-1531	1.000000	89.9
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	GGAAGAGCAATAGGCCAGAC	20	20	50.0	55.0	58.1	58.0	0.0	5.0	0.125805	31472	32824	1509-1532	1.000000	89.9
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTG	20	20	55.0	50.0	58.1	57.9	7.6	0.0	0.177978	31472	32824	1479-1502	1.000000	89.6
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	TTCCTGATGGTTGGTCAAGT	AATCGAACCCGCATCTTCTC	20	20	45.0	50.0	56.7	58.1	0.0	0.0	1.404710	36116	36403	448-450	1.000000	73.6
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	TTCCTGATGGTTGGTCAAGT	GAATCGAACCCGCATCTTCT	20	20	45.0	50.0	56.7	58.1	0.0	0.0	1.404710	36116	36403	449-451	1.000000	73.6
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	TTTCCTGATGGTTGGTCAAGT	AATCGAACCCGCATCTTCTC	21	20	42.9	50.0	57.4	58.1	0.0	0.0	1.652117	36116	36403	449-451	1.000000	71.6
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	TTTCCTGATGGTTGGTCAAGT	GAATCGAACCCGCATCTTCT	21	20	42.9	50.0	57.4	58.1	0.0	0.0	1.652117	36116	36403	450-452	1.000000	71.6
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	TTCCTGATGGTTGGTCAAGT	GAACCCGCATCTTCTCCTTG	20	20	45.0	55.0	56.7	58.6	0.0	0.0	1.972465	36116	36403	444-446	1.000000	69.1
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.961472	44498	44808	483-516	1.000000	74.2
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.963464	44498	44808	482-515	1.000000	74.2
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TGCCTCCTTTTCTCCTGAAG	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.1	58.2	0.0	0.0	1.018457	44498	44808	504-537	1.000000	73.7
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	GCCTCCTTTTCTCCTGAAGT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.1	58.2	0.0	0.0	1.020450	44498	44808	503-536	1.000000	73.7
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	57.1	57.8	0.0	5.6	1.059773	44498	44808	454-487	1.000000	73.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46083	46719	698-760	1.000000	81.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	ATACCAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.1	58.2	33.1	10.2	0.369495	46083	46719	749-811	1.000000	80.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GACTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	58.2	58.2	0.0	10.2	0.406999	46083	46719	729-791	1.000000	80.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46083	46719	692-754	1.000000	79.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATACCAAAAGCCGGCTATCG	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	58.1	57.6	33.1	0.0	0.531832	46083	46719	743-805	1.000000	79.6
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	TGAAATACAAGATGCTCAGTGA	20	22	55.0	36.4	58.0	55.5	10.3	0.0	4.528994	47723	48300	670-704	1.000000	51.9
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCAGTGA	20	22	50.0	36.4	58.1	55.5	0.0	0.0	4.578384	47723	48300	634-668	1.000000	51.5
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CTGGTAGAGCAGAGGACTGA	TGAAATACAAGATGCTCAGTGA	20	22	55.0	36.4	57.9	55.5	2.5	0.0	4.662787	47723	48300	658-692	1.000000	50.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GAATGGTCGGGATAGCTCAG	TGAAATACAAGATGCTCAGTGA	20	22	55.0	36.4	57.6	55.5	0.0	0.0	4.973733	47723	48300	678-712	1.000000	48.3
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	AATCCTCGTGTCACCAGTTC	TGAAATACAAGATGCTCAGTGA	20	22	50.0	36.4	57.5	55.5	0.0	0.0	4.983855	47723	48300	637-671	1.000000	48.3
accD-psaI_p1	accD-psaI	1	TAAGCTCCACGCCTTCTTTC	AAAGAAGCCATCGCAATTGC	20	20	50.0	45.0	57.9	58.0	0.0	20.8	0.095191	57271	57739	553-592	1.000000	90.6
accD-psaI_p2	accD-psaI	2	CTCCACGCCTTCTTTCCTTT	AAAGAAGCCATCGCAATTGC	20	20	50.0	45.0	58.1	58.0	0.0	20.8	0.102869	57271	57739	549-588	1.000000	90.6
accD-psaI_p3	accD-psaI	3	TAAGCTCCACGCCTTCTTTC	TCGCAATTGCCGGAAATACT	20	20	50.0	45.0	57.9	58.3	0.0	24.2	0.349530	57271	57739	543-582	1.000000	88.7
accD-psaI_p4	accD-psaI	4	CTCCACGCCTTCTTTCCTTT	TCGCAATTGCCGGAAATACT	20	20	50.0	45.0	58.1	58.3	0.0	24.2	0.357208	57271	57739	539-578	1.000000	88.7
accD-psaI_p5	accD-psaI	5	CCACGCCTTCTTTCCTTTGA	AAAGAAGCCATCGCAATTGC	20	20	50.0	45.0	58.4	58.0	0.0	20.8	0.395825	57271	57739	547-586	1.000000	88.3
petA-psbJ_p1	petA-psbJ	1	GCGGATTTGGTCAAGGAGAT	ACACAATCAAACCCGAACGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.087826	61373	62182	1333-1518	1.000000	84.3
petA-psbJ_p2	petA-psbJ	2	TCCTAATGTGGGCGGATTTG	ACACAATCAAACCCGAACGA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.095038	61373	62182	1344-1529	1.000000	84.2
petA-psbJ_p3	petA-psbJ	3	TTTATCCCGACGGAAGCAAG	ACACAATCAAACCCGAACGA	20	20	50.0	45.0	58.0	58.0	17.5	0.0	0.062245	61373	62182	1558-1743	1.000000	84.0
petA-psbJ_p4	petA-psbJ	4	CGACGGAAGCAAGAGTAACA	ACACAATCAAACCCGAACGA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.104029	61373	62182	1551-1736	1.000000	83.7
petA-psbJ_p5	petA-psbJ	5	TCCGATCGTATTTCTCCCGA	ACACAATCAAACCCGAACGA	20	20	50.0	45.0	58.0	58.0	15.1	0.0	0.072222	61373	62182	1778-1963	1.000000	83.5
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	55.9	24.9	3.6	2.257324	62960	63839	949-950	1.000000	65.4
psbE-petL_p2	psbE-petL	2	GTTCTCCTGTGCTTCCAGAC	AGCCACTAGTAGAAAACCGA	20	20	55.0	45.0	58.2	55.9	0.9	3.6	2.298912	62960	63839	944-945	1.000000	65.1
psbE-petL_p3	psbE-petL	3	TCTCCTGTGCTTCCAGACAT	AGCCACTAGTAGAAAACCGA	20	20	50.0	45.0	58.4	55.9	0.9	3.6	2.451660	62960	63839	942-943	1.000000	63.9
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACT	20	20	50.0	45.0	57.8	55.7	24.9	0.0	2.445748	62960	63839	996-997	1.000000	63.7
psbE-petL_p5	psbE-petL	5	AGAACGTTCTCCTGTGCTTC	AGCCACTAGTAGAAAACCGAA	20	21	50.0	42.9	57.8	56.7	24.9	3.6	2.477681	62960	63839	949-950	1.000000	63.6
ycf1_p1	ycf1	1	GAGCTACTGAGGAACAACGG	TGAATTCCCGGTAGAAAGCG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.020873	106438	108042	2546-2546	0.333333	53.2
ycf1_p2	ycf1	2	CCCCTTTGGTGTCATTGACA	TGAATTCCCGGTAGAAAGCG	20	20	50.0	50.0	58.0	58.0	15.4	0.0	0.024586	106438	108042	2342-2342	0.333333	53.1
ycf1_p3	ycf1	3	CCCGGAACTTCTTCGTAGTG	TGAATTCCCGGTAGAAAGCG	20	20	55.0	50.0	58.0	58.0	0.7	0.0	0.026372	106438	108042	2451-2451	0.333333	53.1
ycf1_p4	ycf1	4	CCGGAACTTCTTCGTAGTGG	TGAATTCCCGGTAGAAAGCG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.026372	106438	108042	2450-2450	0.333333	53.1
ycf1_p5	ycf1	5	ATGTTTGCTCGCGACAATTG	TGAATTCCCGGTAGAAAGCG	20	20	45.0	50.0	58.0	58.0	19.7	0.0	0.050780	106438	108042	2904-2904	0.333333	52.9
ycf1-rpl32_p1	ycf1-rpl32	1	TAAAGCTTTGGACAGGGCTG	TGAATTCCCGGTAGAAAGCG	20	20	50.0	50.0	58.1	58.0	22.0	0.0	0.108842	108043	108513	1237-1237	0.333333	52.5
ycf1-rpl32_p2	ycf1-rpl32	2	TTGGACAGGGCTGAAATGTC	TGAATTCCCGGTAGAAAGCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.108842	108043	108513	1230-1230	0.333333	52.5
ycf1-rpl32_p3	ycf1-rpl32	3	GACAGGGCTGAAATGTCACA	TGAATTCCCGGTAGAAAGCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.122322	108043	108513	1227-1227	0.333333	52.4
ycf1-rpl32_p4	ycf1-rpl32	4	TTGGACAGGGCTGAAATGTC	CCAAGAACCCTCCCTTTTCC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.165569	108043	108513	1179-1179	0.333333	52.0
ycf1-rpl32_p5	ycf1-rpl32	5	TTGGACAGGGCTGAAATGTC	CCCAAGAACCCTCCCTTTTC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.165569	108043	108513	1180-1180	0.333333	52.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	ACCGGGAATTCAAAAAGTTT	TCCTAAGAGCAGCGTGTCTA	20	20	35.0	50.0	53.9	57.9	6.3	0.0	4.271580	108691	109280	689-715	1.000000	55.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	ACCGGGAATTCAAAAAGTTT	AGCAGCGTGTCTACCAATTT	20	20	35.0	45.0	53.9	57.8	6.3	0.0	4.332307	108691	109280	682-708	1.000000	55.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	ACCGGGAATTCAAAAAGTTT	CCTAAGAGCAGCGTGTCTAC	20	20	35.0	55.0	53.9	57.8	6.3	0.0	4.343598	108691	109280	688-714	1.000000	55.0
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	ACCGGGAATTCAAAAAGTTT	CTAAGAGCAGCGTGTCTACC	20	20	35.0	55.0	53.9	57.8	6.3	0.0	4.343598	108691	109280	687-713	1.000000	55.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ACCGGGAATTCAAAAAGTTT	GAGATGACATGCCGCTACTC	20	20	35.0	55.0	53.9	58.2	6.3	0.0	4.370202	108691	109280	739-765	1.000000	54.2
ccsA-ndhD_p1	ccsA-ndhD	1	TCATCAATTGTGGCGTCGAT	TGCCCGTACTAGGTATTGGT	20	20	45.0	50.0	58.0	57.8	5.1	0.0	0.186574	110415	110653	408-435	1.000000	82.3
ccsA-ndhD_p2	ccsA-ndhD	2	GTTCATCAATTGTGGCGTCG	TGCCCGTACTAGGTATTGGT	20	20	50.0	50.0	58.1	57.8	5.1	0.0	0.281612	110415	110653	410-437	1.000000	81.5
ccsA-ndhD_p3	ccsA-ndhD	3	TTCATCAATTGTGGCGTCGA	TGCCCGTACTAGGTATTGGT	20	20	45.0	50.0	58.2	57.8	5.1	0.0	0.361741	110415	110653	409-436	1.000000	80.9
ccsA-ndhD_p4	ccsA-ndhD	4	AATTGTGGCGTCGATTGGAT	TGCCCGTACTAGGTATTGGT	20	20	45.0	50.0	58.2	57.8	0.0	0.0	0.413617	110415	110653	403-430	1.000000	80.5
ccsA-ndhD_p5	ccsA-ndhD	5	ATTGTGGCGTCGATTGGATT	TGCCCGTACTAGGTATTGGT	20	20	45.0	50.0	58.2	57.8	0.0	0.0	0.413617	110415	110653	402-429	1.000000	80.5
ndhA_p1	ndhA	1	GTGAAAGAAGCTGGGACGAA	TCCAACAGGCTATGGAAGGA	20	20	50.0	50.0	58.1	58.0	0.0	16.5	0.155633	113839	115529	2184-2482	1.000000	83.5
ndhA_p2	ndhA	2	GTGAAAGAAGCTGGGACGAA	CGAGTTGAAGGCATAGGCAT	20	20	50.0	50.0	58.1	58.0	0.0	0.1	0.162005	113839	115529	2404-2702	1.000000	83.2
ndhA_p3	ndhA	3	GTGAAAGAAGCTGGGACGAA	TGGCAAAAAGAGGGGGATTC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.183856	113839	115529	2257-2555	1.000000	83.2
ndhA_p4	ndhA	4	GTGAAAGAAGCTGGGACGAA	CCGGGGTTGCTGAATATCAA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.172381	113839	115529	2457-2755	1.000000	83.0
ndhA_p5	ndhA	5	GTGAAAGAAGCTGGGACGAA	AACCGGGGTTGCTGAATATC	20	20	50.0	50.0	58.1	57.9	0.0	6.3	0.173829	113839	115529	2459-2757	1.000000	83.0