Hotspot

ndhB-trnL(CAA)

IRa · 153939-154692 · 754 bp

Back to Pinellia

Pi 0.0262

MarkerSeek score 77.90

Primer available yes

Label ndhB-trnL(CAA)

Feature Metadata

label_name: ndhB-trnL(CAA)
feature_type: igs
region: IRa
length_bp: 754
variable_sites: 39
pi: 0.0262
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL(CAA)_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	814-941 bp	1.0	87.0
ndhB-trnL(CAA)_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	812-939 bp	1.0	87.0
ndhB-trnL(CAA)_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	809-936 bp	1.0	85.5
ndhB-trnL(CAA)_p4	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	813-940 bp	1.0	84.9
ndhB-trnL(CAA)_p5	TGCAATTGCTCAGGATACCC	TCGCGTGTCTACCATTTCAC	50.0	50.0	57.9	58.3	811-938 bp	1.0	84.7