Hotspot

ndhE-ndhG

SSC · 125979-126351 · 373 bp

Back to Pinellia

Pi 0.128

MarkerSeek score 80.60

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 373
variable_sites: 90
pi: 0.128
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GTGCTCTAACCATGCTTCGA	ATTACTATGGCTCGTCAATAA	50.0	33.3	58.0	52.2	461-1218 bp	1.0	52.0
ndhE-ndhG_p2	TGCTCTAACCATGCTTCGAC	ATTACTATGGCTCGTCAATAA	50.0	33.3	58.0	52.2	460-1217 bp	1.0	52.0
ndhE-ndhG_p3	AGTGCTCTAACCATGCTTCG	ATTACTATGGCTCGTCAATAA	50.0	33.3	58.0	52.2	462-1219 bp	1.0	52.0
ndhE-ndhG_p4	AACCATGCTTCGACTTGTGA	ATTACTATGGCTCGTCAATAA	45.0	33.3	57.7	52.2	454-1211 bp	1.0	52.0
ndhE-ndhG_p5	GCTCTAACCATGCTTCGACT	ATTACTATGGCTCGTCAATAA	50.0	33.3	57.7	52.2	459-1216 bp	1.0	52.0