Hotspot

psaC-ndhE

SSC · 124734-125672 · 939 bp

Back to Pinellia

Pi 0.0709

MarkerSeek score 80.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 939
variable_sites: 123
pi: 0.0709
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TACTGCGAGTTGTCTCATGC	TCGAAGCATGGTTAGAGCAC	50.0	50.0	58.0	58.0	1364-1492 bp	1.0	91.2
psaC-ndhE_p2	GAAGAGCCCGCGAATATAGG	GTCGAAGCATGGTTAGAGCA	55.0	50.0	58.0	58.0	1574-1702 bp	1.0	90.4
psaC-ndhE_p3	GAAGAGCCCGCGAATATAGG	TCGAAGCATGGTTAGAGCAC	55.0	50.0	58.0	58.0	1573-1701 bp	1.0	90.4
psaC-ndhE_p4	GGAAGAGCCCGCGAATATAG	GTCGAAGCATGGTTAGAGCA	55.0	50.0	58.0	58.0	1575-1703 bp	1.0	90.4
psaC-ndhE_p5	GGAAGAGCCCGCGAATATAG	TCGAAGCATGGTTAGAGCAC	55.0	50.0	58.0	58.0	1574-1702 bp	1.0	90.4