Hotspot

ndhF-rpl32

SSC · 119514-120694 · 1181 bp

Back to Pinellia

Pi 0.0586

MarkerSeek score 86.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1181
variable_sites: 143
pi: 0.0586
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGAAAAGGTATTATCCATGCA	TTTGACATAGAAGTACGTTTTT	37.5	27.3	57.0	52.0	1182-1498 bp	1.0	52.0
ndhF-rpl32_p2	GGAAGAAAAGGTATTATCCATGCA	TTTTGACATAGAAGTACGTTTT	37.5	27.3	57.0	52.0	1183-1499 bp	1.0	52.0
ndhF-rpl32_p3	GGAAGAAAAGGTATTATCCATGCA	TTTTTGACATAGAAGTACGTTT	37.5	27.3	57.0	52.0	1184-1500 bp	1.0	52.0
ndhF-rpl32_p4	TGGAAGAAAAGGTATTATCCATGC	TTTGACATAGAAGTACGTTTTT	37.5	27.3	57.0	52.0	1183-1499 bp	1.0	52.0
ndhF-rpl32_p5	TGGAAGAAAAGGTATTATCCATGC	TTTTGACATAGAAGTACGTTTT	37.5	27.3	57.0	52.0	1184-1500 bp	1.0	52.0