Hotspot

trnR(ACG)-trnN(GUU)

SSC · 114683-115279 · 597 bp

Back to Pinellia

Pi 0.0525

MarkerSeek score 97.60

Primer available yes

Label trnR(ACG)-trnN(GUU)

Feature Metadata

label_name: trnR(ACG)-trnN(GUU)
feature_type: igs
region: SSC
length_bp: 597
variable_sites: 74
pi: 0.0525
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 97.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR(ACG)-trnN(GUU)_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	721-776 bp	1.0	90.3
trnR(ACG)-trnN(GUU)_p2	CAGAGGATTAGAGCACGTGG	CACCCGTTGTTCCTCAGTAG	55.0	55.0	57.8	57.9	724-779 bp	1.0	89.6
trnR(ACG)-trnN(GUU)_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	672-727 bp	1.0	89.0
trnR(ACG)-trnN(GUU)_p4	CAGAGGATTAGAGCACGTGG	GAATCACCCGTTGTTCCTCA	55.0	50.0	57.8	57.8	728-783 bp	1.0	88.8
trnR(ACG)-trnN(GUU)_p5	CAGAGGATTAGAGCACGTGG	AATCACCCGTTGTTCCTCAG	55.0	50.0	57.8	57.8	727-782 bp	1.0	88.7