Hotspot

psbE-petL

LSC · 70054-71295 · 1242 bp

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Pi 0.04

MarkerSeek score 80.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1242
variable_sites: 106
pi: 0.04
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACTGACGAATAACCAACCCG	ACCGAAATAACTAGTTATAGTAGGCA	50.0	34.6	57.9	57.5	1371-1588 bp	1.0	50.0
psbE-petL_p2	ACGAATAACCAACCCGCAAT	ACCGAAATAACTAGTTATAGTAGGCA	45.0	34.6	58.2	57.5	1367-1584 bp	1.0	50.0
psbE-petL_p3	ACTGACGAATAACCAACCCG	CCGAAATAACTAGTTATAGTAGGCA	50.0	36.0	57.9	56.0	1370-1587 bp	1.0	50.0
psbE-petL_p4	ACTGACGAATAACCAACCCG	ACCGAAATAACTAGTTATAGTAGGC	50.0	36.0	57.9	56.0	1371-1588 bp	1.0	50.0
psbE-petL_p5	ACGAATAACCAACCCGCAAT	CCGAAATAACTAGTTATAGTAGGCA	45.0	36.0	58.2	56.0	1366-1583 bp	1.0	50.0