Hotspot

trnT(UGU)-trnL(UAA)

LSC · 50012-51231 · 1220 bp

Back to Pinellia

Pi 0.0836
MarkerSeek score 95.40
Primer available yes
Label trnT(UGU)-trnL(UAA)

Feature Metadata

label_name
trnT(UGU)-trnL(UAA)
feature_type
igs
region
LSC
length_bp
1220
variable_sites
194
pi
0.0836
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
95.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(UGU)-trnL(UAA)_p1 GGAAAAAGCCAGCTATCGGA TCCATACCAAGGCTCAATGC 50.0 50.0 58.0 57.9 1267-1548 bp 1.0 91.2
trnT(UGU)-trnL(UAA)_p2 AGGGAAAAAGCCAGCTATCG TCCATACCAAGGCTCAATGC 50.0 50.0 58.0 57.9 1269-1550 bp 1.0 91.2
trnT(UGU)-trnL(UAA)_p3 GGAAAAAGCCAGCTATCGGA ACCAAGGCTCAATGCAATCA 50.0 45.0 58.0 58.1 1262-1543 bp 1.0 91.1
trnT(UGU)-trnL(UAA)_p4 AGGGAAAAAGCCAGCTATCG ACCAAGGCTCAATGCAATCA 50.0 45.0 58.0 58.1 1264-1545 bp 1.0 91.1
trnT(UGU)-trnL(UAA)_p5 GGAAAAAGCCAGCTATCGGA GCGTCTACCTATTTCGCCAT 50.0 50.0 58.0 57.8 1234-1515 bp 1.0 90.5