Hotspot

rps4-trnT(UGU)

LSC · 49472-49938 · 467 bp

Back to Pinellia

Pi 0.0401
MarkerSeek score 80.80
Primer available yes
Label rps4-trnT(UGU)

Feature Metadata

label_name
rps4-trnT(UGU)
feature_type
igs
region
LSC
length_bp
467
variable_sites
35
pi
0.0401
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT(UGU)_p1 CTCCCAGACGGCGTATTTTT GATGGTCATCGGTTCGACTC 50.0 55.0 58.3 58.2 542-938 bp 1.0 85.1
rps4-trnT(UGU)_p2 CTCCCAGACGGCGTATTTTT CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.3 57.9 573-969 bp 1.0 85.0
rps4-trnT(UGU)_p3 CTAGTCAGTCCTGGCAAAGC GATGGTCATCGGTTCGACTC 55.0 55.0 58.3 58.2 561-957 bp 1.0 84.9
rps4-trnT(UGU)_p4 CTAGTCAGTCCTGGCAAAGC CTCAGAGGTTAGAGCATCGC 55.0 55.0 58.3 57.9 592-988 bp 1.0 84.7
rps4-trnT(UGU)_p5 CTCCCAGACGGCGTATTTTT CCGCTTAGCTCAGAGGTTAG 50.0 55.0 58.3 57.5 581-977 bp 1.0 81.9