Hotspot

ycf3-trnS(GGA)

LSC · 47543-48458 · 916 bp

Back to Pinellia

Pi 0.048

MarkerSeek score 85.80

Primer available yes

Label ycf3-trnS(GGA)

Feature Metadata

label_name: ycf3-trnS(GGA)
feature_type: igs
region: LSC
length_bp: 916
variable_sites: 105
pi: 0.048
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS(GGA)_p1	TGGCTACAATCGAAGAGGTC	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.0	58.1	1013-1103 bp	1.0	83.6
ycf3-trnS(GGA)_p2	TGGCTACAATCGAAGAGGTC	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.0	58.2	1034-1124 bp	1.0	83.0
ycf3-trnS(GGA)_p3	TGGCTACAATCGAAGAGGTC	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.0	57.8	984-1074 bp	1.0	82.8
ycf3-trnS(GGA)_p4	TTGGCTACAATCGAAGAGGTC	CCCTCGGTAAACAAAAGCCT	47.6	50.0	57.8	58.1	1014-1104 bp	1.0	81.3
ycf3-trnS(GGA)_p5	TGGCTACAATCGAAGAGGTC	ACCCTCGGTAAACAAAAGCC	50.0	50.0	57.0	58.4	1014-1104 bp	1.0	81.2