Hotspot

rpoB-trnC(GCA)

LSC · 29010-30524 · 1515 bp

Back to Pinellia

Pi 0.0474

MarkerSeek score 88.60

Primer available yes

Label rpoB-trnC(GCA)

Feature Metadata

label_name: rpoB-trnC(GCA)
feature_type: igs
region: LSC
length_bp: 1515
variable_sites: 162
pi: 0.0474
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 88.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC(GCA)_p1	CCCTCATTTTCATCCCGGAG	TGGGGTTTAGATCAGCAGGA	55.0	50.0	58.0	58.0	1612-1830 bp	1.0	91.0
rpoB-trnC(GCA)_p2	CTCATTTTCATCCCGGAGCA	TGGGGTTTAGATCAGCAGGA	50.0	50.0	58.0	58.0	1610-1828 bp	1.0	90.9
rpoB-trnC(GCA)_p3	CCCTCATTTTCATCCCGGAG	GCGACACCCAGATTTGAACT	55.0	50.0	58.0	58.5	1566-1784 bp	1.0	87.7
rpoB-trnC(GCA)_p4	CTCATTTTCATCCCGGAGCA	GCGACACCCAGATTTGAACT	50.0	50.0	58.0	58.5	1564-1782 bp	1.0	87.5
rpoB-trnC(GCA)_p5	CCCTCATTTTCATCCCGGAG	TAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	57.5	1541-1759 bp	1.0	87.4