Hotspot

trnS(GCU)-trnG(UCC)

LSC · 9369-10944 · 1576 bp

Back to Pinellia

Pi 0.142

MarkerSeek score 85.80

Primer available yes

Label trnS(GCU)-trnG(UCC)

Feature Metadata

label_name: trnS(GCU)-trnG(UCC)
feature_type: igs
region: LSC
length_bp: 1576
variable_sites: 402
pi: 0.142
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(GCU)-trnG(UCC)_p1	GAGAAAGCGGAGAAAGAGGG	AGGACGAATCACACTTTTACCA	55.0	40.9	58.0	57.7	1617-1743 bp	1.0	73.1
trnS(GCU)-trnG(UCC)_p2	ATTAGCAATCCGCCGCTTTA	AGGACGAATCACACTTTTACCA	45.0	40.9	58.0	57.7	1562-1688 bp	1.0	72.9
trnS(GCU)-trnG(UCC)_p3	GAGAAAGCGGAGAAAGAGGG	GGACGAATCACACTTTTACCAC	55.0	45.5	58.0	57.6	1616-1742 bp	1.0	72.7
trnS(GCU)-trnG(UCC)_p4	ATTAGCAATCCGCCGCTTTA	GGACGAATCACACTTTTACCAC	45.0	45.5	58.0	57.6	1561-1687 bp	1.0	72.5
trnS(GCU)-trnG(UCC)_p5	AGAAAGCGGAGAAAGAGGGA	AGGACGAATCACACTTTTACCA	50.0	40.9	58.4	57.7	1616-1742 bp	1.0	70.4