Hotspot

trnH-psbA

LSC · 70-718 · 649 bp

Back to Pinellia

Pi 0.108
MarkerSeek score 84.30
Primer available yes
Label trnH-psbA

Feature Metadata

label_name
trnH-psbA
feature_type
igs
region
LSC
length_bp
649
variable_sites
130
pi
0.108
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.95
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-psbA_p1 GATCCACTTGGCTACATCCG AGCTGCTGTTGAAGTTCCAT 55.0 45.0 58.1 57.7 696-714 bp 0.8 80.7
trnH-psbA_p2 CAATCCACTGCCTTGATCCA AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 710-728 bp 0.8 80.7
trnH-psbA_p3 AATCCACTGCCTTGATCCAC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 709-727 bp 0.8 80.7
trnH-psbA_p4 ACAATCCACTGCCTTGATCC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 711-729 bp 0.8 80.7
trnH-psbA_p5 GATCCACTTGGCTACATCCG GCTGCTGTTGAAGTTCCATC 55.0 50.0 58.1 57.4 695-713 bp 0.8 78.0