Hotspot

ycf1

SSC · 125744-131173 · 5430 bp

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Pi 0.00427

MarkerSeek score 49.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5430
variable_sites: 43
pi: 0.00427
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAAGTACAGATCTCAAGTGA	TCGATTTACAACGATGAAACA	38.1	33.3	53.7	53.9	200-233 bp	1.0	46.2
ycf1_p2	TTGAAGTACAGATCTCAAGTGA	TCGATTTACAACGATGAAACA	36.4	33.3	54.5	53.9	201-234 bp	1.0	46.2
ycf1_p3	TGAAGTACAGATCTCAAGTGA	TCGATTTACAACGATGAAACAA	38.1	31.8	53.7	54.7	200-233 bp	1.0	46.2
ycf1_p4	TGAAGTACAGATCTCAAGTGA	TCACAAAATCGATTTACAACGATGA	38.1	32.0	53.7	57.9	208-241 bp	1.0	46.1
ycf1_p5	TGAAGTACAGATCTCAAGTGA	TCACAAAATCGATTTACAACGA	38.1	31.8	53.7	54.7	208-241 bp	1.0	46.1