Hotspot

rpl2

IRa · 154956-156438 · 1483 bp

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Pi 0

MarkerSeek score 26.10

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1483
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GAATGGTTAAAGCGCCCAAC	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.0	58.1	2124 bp	1.0	78.9
rpl2_p2	GAATGGTTAAAGCGCCCAAC	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.0	57.9	2110 bp	1.0	78.9
rpl2_p3	GAATGGTTAAAGCGCCCAAC	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.0	57.9	2109 bp	1.0	78.9
rpl2_p4	GAATGGTTAAAGCGCCCAAC	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.0	57.9	2111 bp	1.0	78.9
rpl2_p5	GCATTGGGTCGAACTCTTCT	CGGATGTAGCCAAGTGGATC	50.0	55.0	57.9	58.1	1795 bp	1.0	78.2