Hotspot

petD

LSC · 78589-79842 · 1254 bp

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Pi 0.00253

MarkerSeek score 60.50

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1254
variable_sites: 6
pi: 0.00253
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GTGGATTTTCCGAAGAGAGGA	TGGCACGATCCGTATTTTCC	47.6	50.0	57.4	58.3	1362-1367 bp	1.0	65.0
petD_p2	AGTGGATTTTCCGAAGAGAGG	TGGCACGATCCGTATTTTCC	47.6	50.0	57.4	58.3	1363-1368 bp	1.0	65.0
petD_p3	GTGGATTTTCCGAAGAGAGGA	AATCTTTGGCACGATCCGTA	47.6	45.0	57.4	57.3	1368-1373 bp	1.0	62.3
petD_p4	GTGGATTTTCCGAAGAGAGGA	TAATCTTTGGCACGATCCGT	47.6	45.0	57.4	57.3	1369-1374 bp	1.0	62.3
petD_p5	GTGGATTTTCCGAAGAGAGGA	TCTTTGGCACGATCCGTATT	47.6	45.0	57.4	57.3	1366-1371 bp	1.0	62.3