Hotspot

psaC-ndhE

SSC · 119219-119669 · 451 bp

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Pi 0.02

MarkerSeek score 72.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 451
variable_sites: 13
pi: 0.02
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	50.0	50.0	58.1	57.4	608-652 bp	1.0	79.6
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.1	57.4	607-651 bp	1.0	79.6
psaC-ndhE_p3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	50.0	50.0	58.1	58.6	628-672 bp	1.0	79.5
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	50.0	55.0	58.1	58.8	606-650 bp	1.0	78.5
psaC-ndhE_p5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	55.0	50.0	58.3	57.4	583-627 bp	1.0	78.2