Hotspot

ndhF-trnL-UAG

SSC · 114686-116009 · 1324 bp

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Pi 0.0256
MarkerSeek score 80.00
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1324
variable_sites
50
pi
0.0256
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 TTGTTGCTGTCGGAATAAGT AGCGTGTCTACCGATTTCAC 40.0 50.0 55.3 58.0 1391-1497 bp 1.0 65.1
ndhF-trnL-UAG_p2 TTGTTGCTGTCGGAATAAGT CAGCGTGTCTACCGATTTCA 40.0 50.0 55.3 58.0 1392-1498 bp 1.0 65.1
ndhF-trnL-UAG_p3 TTGTTGCTGTCGGAATAAGT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 55.3 57.9 1401-1507 bp 1.0 64.1
ndhF-trnL-UAG_p4 TTGTTGCTGTCGGAATAAGT CCTAAGAGCAGCGTGTCTAC 40.0 55.0 55.3 57.8 1400-1506 bp 1.0 63.6
ndhF-trnL-UAG_p5 TTGTTGCTGTCGGAATAAGT CTAAGAGCAGCGTGTCTACC 40.0 55.0 55.3 57.8 1399-1505 bp 1.0 63.6