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trnT-GGU-psbD

LSC · 32632-33694 · 1063 bp

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Pi 0.00737

MarkerSeek score 63.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1063
variable_sites: 15
pi: 0.00737
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AACGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1232-1234 bp	1.0	78.3
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1227-1229 bp	1.0	78.2
trnT-GGU-psbD_p3	AACGGGCCCTTTTAACTCAG	CCCTCCGTAACCAATCATCC	50.0	55.0	58.1	57.7	1216-1218 bp	1.0	78.2
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	CCCTCCGTAACCAATCATCC	50.0	55.0	57.9	57.7	1211-1213 bp	1.0	78.2
trnT-GGU-psbD_p5	AACGGGCCCTTTTAACTCAG	GCCAGACCAGCCTACAAAAA	50.0	50.0	58.1	58.4	1245-1247 bp	1.0	77.2