Hotspot

trnS-GCU-trnS-CGA

LSC · 8307-9666 · 1360 bp

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Pi 0.0574

MarkerSeek score 79.80

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 1360
variable_sites: 116
pi: 0.0574
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	1039-1505 bp	1.0	62.8
trnS-GCU-trnS-CGA_p2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	1042-1508 bp	1.0	61.7
trnS-GCU-trnS-CGA_p3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	1041-1507 bp	1.0	61.7
trnS-GCU-trnS-CGA_p4	AGAAGAAGCGGAGAGAGAGG	ACGAATCACACTTTTACCACT	55.0	38.1	58.2	55.5	1095-1561 bp	1.0	60.9
trnS-GCU-trnS-CGA_p5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	1040-1506 bp	1.0	60.8