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trnK-UUU-rps16

LSC · 4037-4770 · 734 bp

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Pi 0.00432

MarkerSeek score 61.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 6
pi: 0.00432
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCTGGACGAATCTGTTGC	GCTCAACCTACGGGAACTG	50.0	57.9	57.9	57.9	895-901 bp	1.0	71.0
trnK-UUU-rps16_p2	GTCTGGACGAATCTGTTGCT	GCTCAACCTACGGGAACTG	50.0	57.9	57.9	57.9	894-900 bp	1.0	71.0
trnK-UUU-rps16_p3	AGTCTGGACGAATCTGTTGC	GTGCTCAACCTACGGGAAC	50.0	57.9	57.9	58.2	897-903 bp	1.0	70.8
trnK-UUU-rps16_p4	GTCTGGACGAATCTGTTGCT	GTGCTCAACCTACGGGAAC	50.0	57.9	57.9	58.2	896-902 bp	1.0	70.8
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	GCTCAACCTACGGGAACTG	50.0	57.9	58.2	57.9	838-844 bp	1.0	70.4