primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TCAACGATTCATATACACTAAGACA	GTGCTTGGGAGTCCTTGAAA	25	20	32.0	50.0	55.9	58.0	0.0	9.6	7.094328	56	1117	1136-1136	1.000000	42.5
psbA_p2	psbA	2	CAACGATTCATATACACTAAGACA	GTGCTTGGGAGTCCTTGAAA	24	20	33.3	50.0	54.6	58.0	0.0	9.6	7.431279	56	1117	1135-1135	1.000000	42.5
psbA_p3	psbA	3	TCAACGATTCATATACACTAAGACAA	GTGCTTGGGAGTCCTTGAAA	26	20	30.8	50.0	56.6	58.0	0.0	9.6	7.465249	56	1117	1136-1136	1.000000	42.5
psbA_p4	psbA	4	TTCAACGATTCATATACACTAAGACA	GTGCTTGGGAGTCCTTGAAA	26	20	30.8	50.0	56.6	58.0	0.0	9.6	7.465249	56	1117	1137-1137	1.000000	42.5
psbA_p5	psbA	5	TCAACGATTCATATACACTAAGACA	AATACGTGTGCTTGGGAGTC	25	20	32.0	50.0	55.9	57.9	0.0	0.0	7.170346	56	1117	1143-1143	1.000000	42.4
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCTGGACGAATCTGTTGC	GCTCAACCTACGGGAACTG	20	19	50.0	57.9	57.9	57.9	0.0	0.0	1.225083	4037	4770	895-901	1.000000	71.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	GTCTGGACGAATCTGTTGCT	GCTCAACCTACGGGAACTG	20	19	50.0	57.9	57.9	57.9	0.0	0.0	1.225083	4037	4770	894-900	1.000000	71.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AGTCTGGACGAATCTGTTGC	GTGCTCAACCTACGGGAAC	20	19	50.0	57.9	57.9	58.2	0.0	0.0	1.249110	4037	4770	897-903	1.000000	70.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GTCTGGACGAATCTGTTGCT	GTGCTCAACCTACGGGAAC	20	19	50.0	57.9	57.9	58.2	0.0	0.0	1.249110	4037	4770	896-902	1.000000	70.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	GCTCAACCTACGGGAACTG	20	19	50.0	57.9	58.2	57.9	20.3	0.0	1.307449	4037	4770	838-844	1.000000	70.4
trnS-GCU-trnS-CGA_p1	trnS-GCU-trnS-CGA	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	8307	9666	1039-1505	1.000000	62.8
trnS-GCU-trnS-CGA_p2	trnS-GCU-trnS-CGA	2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8307	9666	1042-1508	1.000000	61.7
trnS-GCU-trnS-CGA_p3	trnS-GCU-trnS-CGA	3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8307	9666	1041-1507	1.000000	61.7
trnS-GCU-trnS-CGA_p4	trnS-GCU-trnS-CGA	4	AGAAGAAGCGGAGAGAGAGG	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.2	55.5	0.0	0.0	3.773135	8307	9666	1095-1561	1.000000	60.9
trnS-GCU-trnS-CGA_p5	trnS-GCU-trnS-CGA	5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	20	22	45.0	36.4	58.0	56.2	0.0	0.0	3.814520	8307	9666	1040-1506	1.000000	60.8
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.156700	13738	14277	652-663	1.000000	80.6
atpF-atpH_p2	atpF-atpH	2	CAGTGACCCAAGGAAAGGAA	ATTTATGGACTGGTCGTGGC	20	20	50.0	50.0	57.3	58.0	0.0	0.0	0.695334	13738	14277	624-635	1.000000	76.3
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	GCGTTAGCACTTTTGTTTGC	20	20	50.0	45.0	57.9	57.1	0.0	0.0	1.021669	13738	14277	634-645	1.000000	73.7
atpF-atpH_p4	atpF-atpH	4	CAGTGACCCAAGGAAAGGAAA	ATTTATGGACTGGTCGTGGC	21	20	47.6	50.0	58.1	58.0	0.0	0.0	1.094257	13738	14277	624-635	1.000000	73.2
atpF-atpH_p5	atpF-atpH	5	GTGACCCAAGGAAAGGAAAGA	ATTTATGGACTGGTCGTGGC	21	20	47.6	50.0	57.8	58.0	0.0	0.0	1.262061	13738	14277	622-633	1.000000	71.8
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	20	20	45.0	45.0	58.0	58.6	1.4	16.5	0.624009	14524	15345	911-948	1.000000	75.7
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	20	20	45.0	50.0	58.0	57.4	1.4	21.3	0.625447	14524	15345	883-920	1.000000	75.7
atpH-atpI_p3	atpH-atpI	3	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACGTTAGCCGC	20	20	50.0	45.0	58.0	58.6	3.3	16.5	0.633001	14524	15345	994-1031	1.000000	75.6
atpH-atpI_p4	atpH-atpI	4	TACCTTCTACAGCTTGGCCT	TAGGTGAATCCATGGAGGGT	20	20	50.0	50.0	58.0	57.4	3.3	21.3	0.634438	14524	15345	966-1003	1.000000	75.6
atpH-atpI_p5	atpH-atpI	5	TACCTTGACCAACTCCAGGT	ATTTTTGCAACGTTAGCCGC	20	20	50.0	45.0	57.9	58.6	0.0	16.5	0.697901	14524	15345	967-1004	1.000000	75.1
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	AACGGGCCCTTTTAACTCAG	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	22.4	0.0	0.340432	32632	33694	1232-1234	1.000000	78.3
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.349607	32632	33694	1227-1229	1.000000	78.2
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	AACGGGCCCTTTTAACTCAG	CCCTCCGTAACCAATCATCC	20	20	50.0	55.0	58.1	57.7	22.4	0.0	0.350480	32632	33694	1216-1218	1.000000	78.2
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GCCCTTTTAACTCAGCGGTA	CCCTCCGTAACCAATCATCC	20	20	50.0	55.0	57.9	57.7	0.0	0.0	0.359654	32632	33694	1211-1213	1.000000	78.2
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	AACGGGCCCTTTTAACTCAG	GCCAGACCAGCCTACAAAAA	20	20	50.0	50.0	58.1	58.4	22.4	0.0	0.478204	32632	33694	1245-1247	1.000000	77.2
psbC_p1	psbC	1	AGGGTATTCGTGCTTGGATG	TAGCCGAGCCAGAACAGAAA	20	20	50.0	50.0	57.7	59.0	0.0	0.0	1.358431	34704	36125	1513-1513	1.000000	69.2
psbC_p2	psbC	2	AGGGTATTCGTGCTTGGATG	GCCGAGCCAGAACAGAAAAA	20	20	50.0	50.0	57.7	59.0	0.0	0.0	1.380505	34704	36125	1511-1511	1.000000	69.1
psbC_p3	psbC	3	GAGGGTATTCGTGCTTGGAT	TAGCCGAGCCAGAACAGAAA	20	20	50.0	50.0	57.4	59.0	0.0	0.0	1.648431	34704	36125	1514-1514	1.000000	66.9
psbC_p4	psbC	4	AGGGTATTCGTGCTTGGATG	TAGCCGAGCCAGAACAGAA	20	19	50.0	52.6	57.7	58.3	0.0	0.0	1.665978	34704	36125	1513-1513	1.000000	66.8
psbC_p5	psbC	5	GAGGGTATTCGTGCTTGGAT	GCCGAGCCAGAACAGAAAAA	20	20	50.0	50.0	57.4	59.0	0.0	0.0	1.670506	34704	36125	1512-1512	1.000000	66.7
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	36126	36262	244-244	1.000000	81.9
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CTGTTCTTTCCATGACCCCC	GGTTGATAGCTCCGGTCTTG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.219251	36126	36262	243-243	1.000000	81.9
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.188901	36126	36262	283-283	1.000000	81.6
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	CTGTTCTTTCCATGACCCCC	GATGGCCGAGTGGTTGATAG	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.289496	36126	36262	254-254	1.000000	81.1
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	GCAGCAGGGTTTGAAAAAGG	GATGGCCGAGTGGTTGATAG	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.259146	36126	36262	294-294	1.000000	80.9
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.4	58.1	0.0	0.0	1.678533	45209	45825	780-780	1.000000	67.0
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.4	58.1	0.0	0.0	1.678533	45209	45825	781-781	1.000000	67.0
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	45209	45825	801-801	1.000000	66.5
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	56.4	58.2	0.0	0.0	1.735519	45209	45825	802-802	1.000000	66.5
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	20	20	45.0	45.0	56.4	57.8	0.0	5.6	1.776834	45209	45825	751-751	1.000000	66.2
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	49242	49962	829-840	1.000000	79.5
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	49242	49962	819-830	1.000000	78.9
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGAAATGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	20	20	55.0	50.0	57.8	57.9	0.0	0.0	0.300111	49242	49962	875-886	1.000000	78.5
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	49242	49962	872-883	1.000000	78.2
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	49242	49962	871-882	1.000000	78.2
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.350824	64551	65569	1171-1187	1.000000	79.0
petA-psbJ_p2	petA-psbJ	2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.466931	64551	65569	1167-1183	1.000000	78.1
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.467291	64551	65569	1154-1170	1.000000	78.1
petA-psbJ_p4	petA-psbJ	4	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.468635	64551	65569	1170-1186	1.000000	78.1
petA-psbJ_p5	petA-psbJ	5	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.2	58.3	13.8	0.0	0.491221	64551	65569	1168-1184	1.000000	77.9
petD_p1	petD	1	GTGGATTTTCCGAAGAGAGGA	TGGCACGATCCGTATTTTCC	21	20	47.6	50.0	57.4	58.3	0.0	0.0	1.952772	78589	79842	1362-1367	1.000000	65.0
petD_p2	petD	2	AGTGGATTTTCCGAAGAGAGG	TGGCACGATCCGTATTTTCC	21	20	47.6	50.0	57.4	58.3	0.0	0.0	1.959697	78589	79842	1363-1368	1.000000	65.0
petD_p3	petD	3	GTGGATTTTCCGAAGAGAGGA	AATCTTTGGCACGATCCGTA	21	20	47.6	45.0	57.4	57.3	0.0	0.0	2.295767	78589	79842	1368-1373	1.000000	62.3
petD_p4	petD	4	GTGGATTTTCCGAAGAGAGGA	TAATCTTTGGCACGATCCGT	21	20	47.6	45.0	57.4	57.3	0.0	0.0	2.295767	78589	79842	1369-1374	1.000000	62.3
petD_p5	petD	5	GTGGATTTTCCGAAGAGAGGA	TCTTTGGCACGATCCGTATT	21	20	47.6	45.0	57.4	57.3	0.0	0.0	2.295767	78589	79842	1366-1371	1.000000	62.3
rpl22-rps19_p1	rpl22-rps19	1	CGTCCGCGAATCTGATCAAT	TACTTTCGCGAGACATGCAA	20	20	50.0	45.0	58.5	57.9	8.2	9.6	0.581447	86227	86348	284-307	1.000000	78.3
rpl22-rps19_p2	rpl22-rps19	2	CGTCCGCGAATCTGATCAAT	TTACTTTCGCGAGACATGCA	20	20	50.0	45.0	58.5	57.9	8.2	9.6	0.581447	86227	86348	285-308	1.000000	78.3
rpl22-rps19_p3	rpl22-rps19	3	CGTCCGCGAATCTGATCAAT	GAGAATTCGCGCCTACTCTT	20	20	50.0	50.0	58.5	57.8	8.2	0.0	0.719683	86227	86348	303-326	1.000000	77.0
rpl22-rps19_p4	rpl22-rps19	4	CGTCCGCGAATCTGATCAAT	CAAATTGGGAGAATTCGCGC	20	20	50.0	50.0	58.5	58.5	8.2	17.5	0.959344	86227	86348	311-334	1.000000	75.0
rpl22-rps19_p5	rpl22-rps19	5	CGTCCGCGAATCTGATCAAT	TTCGCGAGACATGCAAGAAA	20	20	50.0	45.0	58.5	58.5	8.2	9.6	1.000768	86227	86348	280-303	1.000000	75.0
ndhF-trnL-UAG_p1	ndhF-trnL-UAG	1	TTGTTGCTGTCGGAATAAGT	AGCGTGTCTACCGATTTCAC	20	20	40.0	50.0	55.3	58.0	0.0	0.0	2.710646	114686	116009	1391-1497	1.000000	65.1
ndhF-trnL-UAG_p2	ndhF-trnL-UAG	2	TTGTTGCTGTCGGAATAAGT	CAGCGTGTCTACCGATTTCA	20	20	40.0	50.0	55.3	58.0	0.0	0.0	2.712016	114686	116009	1392-1498	1.000000	65.1
ndhF-trnL-UAG_p3	ndhF-trnL-UAG	3	TTGTTGCTGTCGGAATAAGT	TCCTAAGAGCAGCGTGTCTA	20	20	40.0	50.0	55.3	57.9	0.0	0.0	2.827093	114686	116009	1401-1507	1.000000	64.1
ndhF-trnL-UAG_p4	ndhF-trnL-UAG	4	TTGTTGCTGTCGGAATAAGT	CCTAAGAGCAGCGTGTCTAC	20	20	40.0	55.0	55.3	57.8	0.0	0.0	2.899111	114686	116009	1400-1506	1.000000	63.6
ndhF-trnL-UAG_p5	ndhF-trnL-UAG	5	TTGTTGCTGTCGGAATAAGT	CTAAGAGCAGCGTGTCTACC	20	20	40.0	55.0	55.3	57.8	0.0	0.0	2.899111	114686	116009	1399-1505	1.000000	63.6
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	119219	119669	608-652	1.000000	79.6
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	119219	119669	607-651	1.000000	79.6
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	20	20	50.0	50.0	58.1	58.6	0.0	12.2	0.757229	119219	119669	628-672	1.000000	79.5
psaC-ndhE_p4	psaC-ndhE	4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	119219	119669	606-650	1.000000	78.5
psaC-ndhE_p5	psaC-ndhE	5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	20	20	55.0	50.0	58.3	57.4	0.0	0.0	0.943571	119219	119669	583-627	1.000000	78.2
ycf1_p1	ycf1	1	TGAAGTACAGATCTCAAGTGA	TCGATTTACAACGATGAAACA	21	21	38.1	33.3	53.7	53.9	0.0	0.0	10.406902	127705	127824	200-233	1.000000	46.2
ycf1_p2	ycf1	2	TTGAAGTACAGATCTCAAGTGA	TCGATTTACAACGATGAAACA	22	21	36.4	33.3	54.5	53.9	0.0	0.0	10.558772	127705	127824	201-234	1.000000	46.2
ycf1_p3	ycf1	3	TGAAGTACAGATCTCAAGTGA	TCGATTTACAACGATGAAACAA	21	22	38.1	31.8	53.7	54.7	0.0	0.0	10.589639	127705	127824	200-233	1.000000	46.2
ycf1_p4	ycf1	4	TGAAGTACAGATCTCAAGTGA	TCACAAAATCGATTTACAACGATGA	21	25	38.1	32.0	53.7	57.9	0.0	23.0	10.442890	127705	127824	208-241	1.000000	46.1
ycf1_p5	ycf1	5	TGAAGTACAGATCTCAAGTGA	TCACAAAATCGATTTACAACGA	21	22	38.1	31.8	53.7	54.7	0.0	23.0	10.589639	127705	127824	208-241	1.000000	46.1
rpl2_p1	rpl2	1	GAATGGTTAAAGCGCCCAAC	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	58.0	58.1	2.3	0.2	0.138121	154956	156438	2124-2124	1.000000	78.9
rpl2_p2	rpl2	2	GAATGGTTAAAGCGCCCAAC	TGGATCAAGGCAGTGGATTG	20	20	50.0	50.0	58.0	57.9	2.3	0.0	0.142482	154956	156438	2110-2110	1.000000	78.9
rpl2_p3	rpl2	3	GAATGGTTAAAGCGCCCAAC	GGATCAAGGCAGTGGATTGT	20	20	50.0	50.0	58.0	57.9	2.3	0.0	0.143990	154956	156438	2109-2109	1.000000	78.9
rpl2_p4	rpl2	4	GAATGGTTAAAGCGCCCAAC	GTGGATCAAGGCAGTGGATT	20	20	50.0	50.0	58.0	57.9	2.3	0.0	0.143990	154956	156438	2111-2111	1.000000	78.9
rpl2_p5	rpl2	5	GCATTGGGTCGAACTCTTCT	CGGATGTAGCCAAGTGGATC	20	20	50.0	55.0	57.9	58.1	0.0	0.2	0.229524	154956	156438	1795-1795	1.000000	78.2