Hotspot

rpl32-trnL-UAG

SSC · 120749-121410 · 662 bp

Back to Pimpinella

Pi 0.0739

MarkerSeek score 69.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 662
variable_sites: 138
pi: 0.0739
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TACTGGGCAGCCTTAAAAGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.2	58.0	722-1073 bp	1.0	90.6
rpl32-trnL-UAG_p2	TACTGGGCAGCCTTAAAAGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.2	58.0	723-1074 bp	1.0	90.6
rpl32-trnL-UAG_p3	TACTGGGCAGCCTTAAAAGC	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.2	58.1	1214-1555 bp	1.0	90.0
rpl32-trnL-UAG_p4	TACTGGGCAGCCTTAAAAGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	732-1083 bp	1.0	89.7
rpl32-trnL-UAG_p5	TACTGGGCAGCCTTAAAAGC	TGAAGTAGCAAAGCCTTGGG	50.0	50.0	58.2	58.1	1215-1548 bp	0.423077	66.9