Hotspot

ndhF-rpl32

SSC · 119421-120583 · 1163 bp

Back to Pimpinella

Pi 0.051

MarkerSeek score 74.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1163
variable_sites: 186
pi: 0.051
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCGTGTGAACCAAAACCCTA	TAAGGCTGCCCAGTATCCTT	50.0	50.0	58.0	58.1	1754-1780 bp	0.230769	51.9
ndhF-rpl32_p2	CCGTGTGAACCAAAACCCTA	AACTAAAACAGGGCTCCAGC	50.0	50.0	58.0	58.1	2067-2087 bp	0.115385	44.0
ndhF-rpl32_p3	GTTGCCGACGGAAAAAGAAG	AACTAAAACAGGGCTCCAGC	50.0	50.0	58.0	58.1	1661-1681 bp	0.115385	44.0
ndhF-rpl32_p4	TGCCGACGGAAAAAGAAGAA	AACTAAAACAGGGCTCCAGC	45.0	50.0	58.0	58.1	1659-1679 bp	0.115385	43.9
ndhF-rpl32_p5	TTGCCGACGGAAAAAGAAGA	AACTAAAACAGGGCTCCAGC	45.0	50.0	58.0	58.1	1660-1680 bp	0.115385	43.9