Hotspot

petA-psbJ

LSC · 69627-70597 · 971 bp

Back to Pimpinella

Pi 0.0593

MarkerSeek score 75.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 971
variable_sites: 162
pi: 0.0593
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAGGGCCAGAACTTCTTGTT	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1240-1799 bp	1.0	91.9
petA-psbJ_p2	CAGGGCCAGAACTTCTTGTT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1239-1798 bp	1.0	91.9
petA-psbJ_p3	CGGATTTGGTCAGGGAGATG	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.0	58.0	1164-1723 bp	1.0	91.6
petA-psbJ_p4	CGGATTTGGTCAGGGAGATG	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	1163-1722 bp	1.0	91.6
petA-psbJ_p5	TCCTAATGTGGGCGGATTTG	ACTGGCCGTTTTGATCCTTT	50.0	45.0	57.9	58.0	1175-1734 bp	1.0	91.6