Hotspot

atpB-rbcL

LSC · 60818-61581 · 764 bp

Back to Pimpinella

Pi 0.0336

MarkerSeek score 71.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 81
pi: 0.0336
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGGTTCATTCATCTGACCGT	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1701-1732 bp	1.0	88.7
atpB-rbcL_p2	CGGTTCATTCATCTGACCGT	GGGGACGACCATACTTGTTC	50.0	55.0	58.0	58.0	1982-2013 bp	1.0	87.8
atpB-rbcL_p3	CGGTTCATTCATCTGACCGT	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2132-2163 bp	1.0	87.5
atpB-rbcL_p4	CGGTTCATTCATCTGACCGT	GTACCCGCAGTAGCATTCAA	50.0	50.0	58.0	58.0	2214-2245 bp	1.0	87.2
atpB-rbcL_p5	ACCCCAGAACTAGAAGCAGT	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	1454-1462 bp	0.115385	44.8