Hotspot

rps4-trnT-UGU

LSC · 52756-53128 · 373 bp

Back to Pimpinella

Pi 0.0496
MarkerSeek score 72.40
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
373
variable_sites
63
pi
0.0496
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.91
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 GGTTTAGGTCTGACGCGAAT TCAGAGCGCAAGAATGTCTC 50.0 50.0 58.0 58.0 1389-1525 bp 0.230769 56.3
rps4-trnT-UGU_p2 GTCCGAACCCCTGTGATTTT TCAGAGCGCAAGAATGTCTC 50.0 50.0 58.1 58.0 1421-1562 bp 0.230769 55.6
rps4-trnT-UGU_p3 AAAACCTGACCTGTTGACCC TCAGAGCGCAAGAATGTCTC 50.0 50.0 57.9 58.0 1055-1164 bp 0.153846 53.0
rps4-trnT-UGU_p4 GGTTTAGGTCTGACGCGAAT ACGAATCAGAGCGCAAGAAT 50.0 45.0 58.0 58.0 1514-1530 bp 0.115385 45.0
rps4-trnT-UGU_p5 AAAACCTGACCTGTTGACCC ACGAATCAGAGCGCAAGAAT 50.0 45.0 57.9 58.0 1153-1169 bp 0.115385 44.6