Hotspot

ycf3-trnS-GGA

LSC · 50930-51796 · 867 bp

Back to Pimpinella

Pi 0.0423

MarkerSeek score 71.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 114
pi: 0.0423
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	GTGGCCTATTGGTACTGGTG	ATTCGCGTCAGACCTAAACC	55.0	50.0	58.0	58.0	1618-1675 bp	0.307692	60.4
ycf3-trnS-GGA_p2	GTGCTTCTTCCCCTATGTGG	ATTCGCGTCAGACCTAAACC	55.0	50.0	58.0	58.0	1645-1691 bp	0.230769	52.8
ycf3-trnS-GGA_p3	TCCGAGGTTGCATTAATGGG	ATTCGCGTCAGACCTAAACC	50.0	50.0	57.9	58.0	1529-1538 bp	0.115385	44.3
ycf3-trnS-GGA_p4	GGTACTGGTGGAGTAGGGTT	ATTCGCGTCAGACCTAAACC	55.0	50.0	58.1	58.0	1619-1628 bp	0.115385	44.3
ycf3-trnS-GGA_p5	TTGGTACTGGTGGAGTAGGG	ATTCGCGTCAGACCTAAACC	55.0	50.0	58.1	58.0	1621-1630 bp	0.115385	44.3