Hotspot

trnE-UUC-trnT-GGU

LSC · 36844-37739 · 896 bp

Back to Pimpinella

Pi 0.0592

MarkerSeek score 77.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 896
variable_sites: 162
pi: 0.0592
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AAACCACTAGACGATGGGGG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	59.1	57.9	639-937 bp	1.0	82.4
trnE-UUC-trnT-GGU_p2	AACCACTAGACGATGGGGG	TACCGCTGAGTTAAAAGGGC	57.9	50.0	58.4	57.9	638-936 bp	1.0	80.0
trnE-UUC-trnT-GGU_p3	CCTAAACCACTAGACGATGGG	TACCGCTGAGTTAAAAGGGC	52.4	50.0	57.5	57.9	642-940 bp	1.0	79.5
trnE-UUC-trnT-GGU_p4	CTAAACCACTAGACGATGGGG	TACCGCTGAGTTAAAAGGGC	52.4	50.0	57.5	57.9	641-939 bp	1.0	79.5
trnE-UUC-trnT-GGU_p5	TAAACCACTAGACGATGGGG	TACCGCTGAGTTAAAAGGGC	50.0	50.0	56.3	57.9	640-938 bp	1.0	77.9