Hotspot

rps16-trnQ-UUG

LSC · 11352-12700 · 1349 bp

Back to Pimpinella

Pi 0.0491
MarkerSeek score 67.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1349
variable_sites
182
pi
0.0491
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.63
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TTCTACCCGAGCTCCATCAT CTTACAGCAGCTTGCCAAAC 50.0 50.0 57.9 57.9 1616-2159 bp 1.0 90.8
rps16-trnQ-UUG_p2 ACTTTCTACCCGAGCTCCAT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.1 57.9 1619-2162 bp 1.0 90.5
rps16-trnQ-UUG_p3 TTCTACCCGAGCTCCATCAT TTTGCCCTGGAATTGACCAA 50.0 45.0 57.9 57.9 1157-1700 bp 0.230769 54.4
rps16-trnQ-UUG_p4 TTCTACCCGAGCTCCATCAT CCCAGCTATCCAACGAAGAC 50.0 55.0 57.9 58.1 1754-2297 bp 0.230769 54.3
rps16-trnQ-UUG_p5 ACTTTCTACCCGAGCTCCAT CCCAGCTATCCAACGAAGAC 50.0 55.0 58.1 58.1 1757-2300 bp 0.230769 53.9