Hotspot

trnK-UUU-rps16

LSC · 9351-10246 · 896 bp

Back to Pimpinella

Pi 0.0641
MarkerSeek score 70.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
896
variable_sites
154
pi
0.0641
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.58
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG TCTATCCCAATGAGCCGTCT 55.0 50.0 58.0 57.9 836-1244 bp 1.0 91.2
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG TCTATCCCAATGAGCCGTCT 55.0 50.0 58.0 57.9 835-1243 bp 1.0 91.2
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG AGCCGTCTATCGAATCGTTG 55.0 50.0 58.0 57.9 824-1232 bp 1.0 90.9
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG AGCCGTCTATCGAATCGTTG 55.0 50.0 58.0 57.9 823-1231 bp 1.0 90.9
trnK-UUU-rps16_p5 TCCCTCGCTTCATCCAAATG TCTATCCCAATGAGCCGTCT 50.0 50.0 58.0 57.9 988-1192 bp 0.346154 64.8