Hotspot

psbA-trnK-UUU

LSC · 6542-6754 · 213 bp

Back to Pimpinella

Pi 0.0501

MarkerSeek score 73.00

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 213
variable_sites: 36
pi: 0.0501
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	GCTTTCGCGTCTCTCTAAAA	CTAGTTCCGGGTTCGAATCC	45.0	55.0	56.2	57.8	260-297 bp	1.0	75.9
psbA-trnK-UUU_p2	TCGCGTCTCTCTAAAATAGCAG	CTAGTTCCGGGTTCGAATCC	45.5	55.0	58.0	57.8	256-293 bp	1.0	73.9
psbA-trnK-UUU_p3	TCGCGTCTCTCTAAAATAGCA	CTAGTTCCGGGTTCGAATCC	42.9	55.0	57.0	57.8	256-293 bp	1.0	73.8
psbA-trnK-UUU_p4	GCTTTCGCGTCTCTCTAAAA	ACTCCATCCGACTAGTTCCG	45.0	55.0	56.2	58.6	271-308 bp	1.0	72.8
psbA-trnK-UUU_p5	GCTTTCGCGTCTCTCTAAAAT	CTAGTTCCGGGTTCGAATCC	42.9	55.0	56.7	57.8	260-297 bp	1.0	72.1