Hotspot

rpl32-trnL

SSC · 113274-114123 · 850 bp

Back to Pilosella

Pi 0.00959

MarkerSeek score 46.00

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 850
variable_sites: 20
pi: 0.00959
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	AAAGGAAGGGATATTGGGCG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	926-983 bp	1.0	82.8
rpl32-trnL_p2	AAAGGAAGGGATATTGGGCG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	927-984 bp	1.0	82.8
rpl32-trnL_p3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.6	58.0	908-965 bp	1.0	82.7
rpl32-trnL_p4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.6	58.0	909-966 bp	1.0	82.6
rpl32-trnL_p5	AAAGGAAGGGATATTGGGCG	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	57.6	58.1	1428-1485 bp	1.0	81.5