Hotspot

ndhF-rpl32

SSC · 111981-113108 · 1128 bp

Back to Pilosella

Pi 0.00679

MarkerSeek score 52.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1128
variable_sites: 19
pi: 0.00679
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CGCCCAATATCCCTTCCTTT	50.0	50.0	58.0	57.6	1276-1307 bp	1.0	80.0
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CGCCCAATATCCCTTCCTTT	45.0	50.0	58.0	57.6	1274-1305 bp	1.0	79.9
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CGCCCAATATCCCTTCCTTT	45.0	50.0	58.0	57.6	1275-1306 bp	1.0	79.9
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	50.0	45.0	58.0	57.6	1294-1325 bp	1.0	79.7
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	45.0	45.0	58.0	57.6	1292-1323 bp	1.0	79.6