Hotspot

rps12-ycf15

IRb · 98739-99721 · 983 bp

Back to Pilosella

Pi 0.000831
MarkerSeek score 53.70
Primer available yes
Label rps12-ycf15

Feature Metadata

label_name
rps12-ycf15
feature_type
igs
region
IRb
length_bp
983
variable_sites
2
pi
0.000831
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-ycf15_p1 TGGCAACTTTACGTAAGGCA GAAAGGACTCACTAAGCCGG 45.0 55.0 57.7 58.0 1038-1058 bp 1.0 79.5
rps12-ycf15_p2 TGGCAACTTTACGTAAGGCA CACGGTACAATCCTCTTCCC 45.0 55.0 57.7 57.7 1127-1147 bp 1.0 77.0
rps12-ycf15_p3 ACGTAAGGCAGAGTTTGGTT GAAAGGACTCACTAAGCCGG 45.0 55.0 57.4 58.0 1028-1048 bp 1.0 76.5
rps12-ycf15_p4 TGGCAACTTTACGTAAGGCA TCACTAAGCCGGGATCACTA 45.0 50.0 57.7 57.6 1030-1050 bp 1.0 76.1
rps12-ycf15_p5 TGGCAACTTTACGTAAGGCA CTCGGTCCACAGAGACAAAA 45.0 50.0 57.7 57.5 1090-1110 bp 1.0 75.3