Hotspot

petA-psbJ

LSC · 63469-64305 · 837 bp

Back to Pilosella

Pi 0.0103

MarkerSeek score 58.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 837
variable_sites: 14
pi: 0.0103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.0	57.6	954-975 bp	1.0	81.9
petA-psbJ_p2	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	45.0	50.0	58.0	57.4	992-1013 bp	1.0	80.2
petA-psbJ_p3	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	45.0	50.0	58.0	57.4	991-1012 bp	1.0	80.1
petA-psbJ_p4	TCCATTACGTGTACAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	960-981 bp	1.0	79.1
petA-psbJ_p5	CCATTACGTGTACAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.6	57.6	959-980 bp	1.0	79.1