Hotspot

ycf3-trnS

LSC · 44401-45312 · 912 bp

Back to Pilosella

Pi 0.00655

MarkerSeek score 53.10

Primer available yes

Label ycf3-trnS

Feature Metadata

label_name: ycf3-trnS
feature_type: igs
region: LSC
length_bp: 912
variable_sites: 14
pi: 0.00655
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS_p1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	969-1022 bp	1.0	75.6
ycf3-trnS_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	919-972 bp	1.0	75.3
ycf3-trnS_p3	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	47.6	55.0	58.0	57.8	957-1010 bp	1.0	75.0
ycf3-trnS_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	921-974 bp	1.0	74.8
ycf3-trnS_p5	CATTTATCCGCGATCTAGGCA	CCTCGGTAAGCAAAAGCCTA	47.6	50.0	58.0	57.6	947-1000 bp	1.0	73.7