Hotspot

psbM-trnD-GUC

LSC · 11003-11680 · 678 bp

Back to Pilosella

Pi 0.0106

MarkerSeek score 50.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 18
pi: 0.0106
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.6	58.3	782-796 bp	1.0	53.6
psbM-trnD-GUC_p2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	40.0	50.0	54.5	58.3	776-790 bp	1.0	53.2
psbM-trnD-GUC_p3	GGAACTAAAATGAACAGTGCAGT	TCCCCTGGGATTGTAGTTCA	39.1	50.0	57.6	57.6	798-812 bp	1.0	52.8
psbM-trnD-GUC_p4	AAAATGAACAGTGCAGTAGC	TCCCCTGGGATTGTAGTTCA	40.0	50.0	54.5	57.6	792-806 bp	1.0	52.4
psbM-trnD-GUC_p5	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.4	58.3	779-793 bp	1.0	52.2