Hotspot

trnC-GCA-petN

LSC · 9494-10331 · 838 bp

Back to Pilosella

Pi 0.0105

MarkerSeek score 46.10

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 22
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	894-957 bp	1.0	81.1
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	912-975 bp	1.0	78.8
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	893-956 bp	1.0	75.1
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	902-965 bp	1.0	75.0
trnC-GCA-petN_p5	TCAGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.6	58.2	951-1014 bp	0.8	66.3