Hotspot

rps16

LSC · 4877-6054 · 1178 bp

Pi 0.0508

MarkerSeek score 81.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1178
variable_sites: 216
pi: 0.0508
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACGGCGCAATCAAATCAAAG	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	2198-2536 bp	0.1875	52.7
rps16_p2	TGGCACTACATATCGAAGCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1853-2199 bp	0.1875	51.8
rps16_p3	ACGGCGCAATCAAATCAAAG	GCGACTTCGTCAGAAACTCT	45.0	50.0	58.0	57.9	1948-1971 bp	0.1875	48.7
rps16_p4	TGGCACTACATATCGAAGCG	GCGACTTCGTCAGAAACTCT	50.0	50.0	57.9	57.9	1611-1625 bp	0.1875	47.6
rps16_p5	ACGGCGCAATCAAATCAAAG	TTCCGTCCCAGAGCATATCT	45.0	50.0	58.0	57.9	2523 bp	0.0625	41.6